Neural Stem Cell Markers

Neural stem cells (NSCs) offer a unique and powerful tool for basic research and regenerative medicine. Changes in the expression levels of specific molecules help to identify the presence of neural stem cells in studies focused on further differentiation toward specific neural lineages.

Commonly used markers for neural stem cells include Nestin and SOX2. Other protein markers, expressed at the cell surface are, for example, ABCG2, FGFR1 and Frizzled-9.

Neurons

The neurons (nerve cells) are electrically excitable cells that transmit and relay the nerve signal within the central nervous system and in the periphery.

The neurons communicate via synapses and fall into different functional categories based on their neurochemical properties.

Figure 1:Representative staining of Neurofilament using monoclonal Anti-NEFL antibody (AMAb91314). (A) The human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells. (B) Immunofluorescence staining of mouse striatum shows strong positivity in neural fibers

Products for Neural Stem Cell Markers

AMAb90595

AMAb90595

Target: BLBp/FABP7

Clonality: Monoclonal

Validated Application: IHC, WB

Sequence Identity Mouse/Rat: 89%/90%

 

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HPA030472

HPA030472

Target: BMI

Clonality: Polyclonal

Validated Application: IHC, WB*

Sequence Identity Mouse/Rat: 95%/95%

 

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HPA004922

HPA004922

Target: CD133/PROML1A

Clonality: Polyclonal

Validated Application: IHC*

Sequence Identity Mouse/Rat: 57%/60%

 

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AMAb90819

AMAb90819

Target: EGFR/ERBB1

Clonality: Monoclonal

Validated Application: WB

Sequence Identity Mouse/Rat: 90%/91%

 

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HPA029937

HPA029937

Target: EGR1/INGFI-A

Clonality: Polyclonal

Validated Application: ICC-IF

Sequence Identity Mouse/Rat: 93%/94%

 

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HPA038349

HPA038349

Target:L PCGF5

Clonality: Polyclonal

Validated Application: IHC, ICC-IDF

Sequence Identity Mouse/Rat: 95%/93%

 

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HPA046512

HPA046512

Target: REELIN/RELN

Clonality: Polyclonal

Validated Application: ICC-IF

Sequence Identity Mouse/Rat: 90%/94%

 

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HPA037468

HPA037468

Target: SLC1A3/GLAST

Clonality: Polyclonal

Validated Application: IHC*

Sequence Identity Mouse/Rat: 93%/93%

 

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HPA055642

HPA055642

Target: TLX/NR2E1

Clonality: Polyclonal

Validated Application: IHC*

Sequence Identity Mouse/Rat: 99%/99%

 

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*Products with enhanced validation for indicated application

Astrocytes

Astrocytes constitute the main glial cell population in the central nervous system. These cells are derived from heterogeneous populations of progenitor cells in the neuroepithelium of the developing central nervous system.

Astrocytes play a number of active roles in the brain, including the secretion or absorption of neural transmitters, ion homeostasis and maintenance of the blood–brain barrier.

Figure 3: Representative staining of Glial Fibrillary Acidic Protein, GFAP, using Anti-GFAP monoclonal antibody (AMAb91033). (A) Immunohistochemical staining of the human cerebral cortex shows strong cytoplasmic positivity in astrocytes. (B) Immunofluorescence staining of rat hippocampus shows strong positivity of the astrocytes.

Products for Astroglial Markers

AMAb90537

AMAb90537

Target: AQP4

Clonality: Monoclonal

Validated Application: IHC*, WB

Sequence Identity Mouse/Rat: 93%/93%

 

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HPA014784

HPA014784

Target: AQP4

Clonality: Polyclonal

Validated Application: IHC*, WB, ICC-IF

Sequence Identity Mouse/Rat: 93%/92%

 

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HPA074762

HPA074762

Target: AQP9

Clonality: Polyclonal

Validated Application: IHC*

Sequence Identity Mouse/Rat: 55%/55%

 

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HPA005785

HPA005785

Target: CD44

Clonality: Polyclonal

Validated Application: IHC*, WB*, ICC-IF

Sequence Identity Mouse/Rat: 51%/47%

 

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AMAb91033

AMAb91033

Target: GFAP

Clonality: Monoclonal

Validated Application: IHC*, WB*

Sequence Identity Mouse/Rat: 98%/100%

 

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HPA056030

HPA056030

Target: GFAP

Clonality: Polyclonal

Validated Application: IHC*, WB, ICC-IF

Sequence Identity Mouse/Rat: 98%/100%

 

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*Products with enhanced validation for indicated application

Oligodendrocytes

Oligodendrocytes are glial cells within the CNS and spinal cord whose main function

is to form the insulating myelin sheaths around axons in the CNS. Furthermore, oligodendrocytes provide trophic support to neurons by the production of different growth factors like BDNF and GDNF.

Figure 2: NSCs primarily differentiate into neurons, astrocytes, and oligodendrocytes, depending on environmental cues.

 

Product for Oligodendroglial Markers

HPA004947

HPA004947

Target: CD140/PDGFRA

Clonality: Polyclonal

Validated Application: ICC-IF

Sequence Identity Mouse/Rat: -

 

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HPA004922

HPA004922

Target: CD133/PROML1A

Clonality: Polyclonal

Validated Application: IHC*

Sequence Identity Mouse/Rat: 57%/60%

 

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HPA003254

HPA003254

Target: OLIG2

Clonality: Polyclonal

Validated Application: IHC*, WB

Sequence Identity Mouse/Rat: 93%/94%

 

 

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HPA000842

HPA000842

Target: PROX1

Clonality: Polyclonal

Validated Application: ICC-IF

Sequence Identity Mouse/Rat: 100%/93%

 

 

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HPA001030

HPA001030

Target: PROX1

Clonality: Polyclonal

Validated Application: ICC-IF

Sequence Identity Mouse/Rat: 100%/99%

 

 

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AMAb91297

AMAb91297

Target: SOX10

Clonality: Monoclonal

Validated Application: IHC, ICC-IF

Sequence Identity Mouse/Rat: 98%/98%

 

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HPA058665

HPA058665

Target: SOX8

Clonality: Polyclonal

Validated Application: IHC*, ICC-IF

Sequence Identity Mouse/Rat: 73%/70%

 

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*Products with enhanced validation for indicated application

Products for Neuronal Stem Cell Markers

AMAb91406

AMAb91406

Target: BRN2/POU3F2

Clonality: Monoclonal

Validated Applicstion: IHC, WB, ICC-IF

Sequence Identity Mouse/Rat: 100%/100%

 

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HPA056261

HPA056261

Target: BRN2/POU3F2

Clonality: Polyclonal

Validated Application: ICC-IF

Sequence Identity Mouse/Rat: 100%/100%

 

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HPA056402

HPA056402

Target: FGFR1

Clonality: Polyclonal

Validated Application: IHC. WB

Sequence Identity Mouse/Rat: 94%/96%

 

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HPA050505

HPA050505

Target: FOXA2

Clonality: Polyclonal

Validated Application: IHC*, ICC-IF

Sequence Identity Mouse/Rat: 96%/82%

 

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AMAb91375

AMAb91375

Target: MAP2

Clonality: Monoclonal

Validated Application: IHC, WB, ICC-IF

Sequence Identity Mouse/Rat: 91%/90%

 

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AMAb91375

AMAb91375

Target: NEFL

Clonality: MOnoclonal

Validated Application: IHC, WB, ICC-IF

Sequence Identity Mouse/Rat: 97%/99%

 

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*Products with enhanced validation for indicated application

Related Publications

Bond AM. (2015) Adult Mammalian Neural Stem Cells and Neurogenesis: Five Decades Later. Cell Stem Cell. 1;17(4):385-95

Casarosa S. et al. (2014) Neural stem cells: ready for therapeutic applications? Mol Cell Ther. 2: 31

Gage FH.,Temple S. (2013) Neural Stem Cells: Generating and Regenerating the Brain. Neuron 80 (3):588-601

Jessell TM. et al. (2000) Principles of Neural Science, 4th Edition. New York:McGraw-Hill