Neural Stem Cell Markers
Neural stem cells (NSCs) offer a unique and powerful tool for basic research and regenerative medicine. Changes in the expression levels of specific molecules help identify the presence of neural stem cells in studies focused on further differentiation toward distinct neural lineages.
Commonly used markers for neural stem cells include Nestin and SOX2. Other protein markers expressed at the cell surface are, for example, ABCG2, FGFR1, and Frizzled-9.
Neurons
The neurons (nerve cells) are electrically excitable cells that transmit and relay the nerve signal within the central nervous system and periphery.
The neurons communicate via synapses and fall into different functional categories based on their neurochemical properties.
Figure 1:Representative staining of Neurofilament using monoclonal Anti-NEFL antibody (AMAb91314). (A) The human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells. (B) Immunofluorescence staining of mouse striatum shows strong positivity in neural fibers
Products for Neural Stem Cell Markers
AMAb90595
Target: BLBp/FABP7
Clonality: Monoclonal
Validated Application: IHC, WB
Sequence Identity Mouse/Rat: 89%/90%
HPA030472
Target: BMI
Clonality: Polyclonal
Validated Application: IHC, WB*
Sequence Identity Mouse/Rat: 95%/95%
HPA004922
Target: CD133/PROML1A
Clonality: Polyclonal
Validated Application: IHC*
Sequence Identity Mouse/Rat: 57%/60%
AMAb90819
Target: EGFR/ERBB1
Clonality: Monoclonal
Validated Application: WB
Sequence Identity Mouse/Rat: 90%/91%
HPA029937
Target: EGR1/INGFI-A
Clonality: Polyclonal
Validated Application: ICC-IF
Sequence Identity Mouse/Rat: 93%/94%
HPA038349
Target:L PCGF5
Clonality: Polyclonal
Validated Application: IHC, ICC-IDF
Sequence Identity Mouse/Rat: 95%/93%
HPA046512
Target: REELIN/RELN
Clonality: Polyclonal
Validated Application: ICC-IF
Sequence Identity Mouse/Rat: 90%/94%
HPA037468
Target: SLC1A3/GLAST
Clonality: Polyclonal
Validated Application: IHC*
Sequence Identity Mouse/Rat: 93%/93%
HPA055642
Target: TLX/NR2E1
Clonality: Polyclonal
Validated Application: IHC*
Sequence Identity Mouse/Rat: 99%/99%
*Products with enhanced validation for the indicated application
Figure 2: NSCs primarily differentiate into neurons, astrocytes, and oligodendrocytes, depending on environmental cues.
Astrocytes
Astrocytes constitute the principal glial cell population in the central nervous system. These cells are derived from heterogeneous populations of progenitor cells in the neuroepithelium of the developing central nervous system.
Astrocytes play several active roles in the brain, including the secretion or absorption of neural transmitters, ion homeostasis, and maintenance of the blood-brain barrier.
Figure 3: Representative staining of Glial Fibrillary Acidic Protein, GFAP, using Anti-GFAP monoclonal antibody (AMAb91033). (A) Immunohistochemical staining of the human cerebral cortex shows strong cytoplasmic positivity in astrocytes. (B) Immunofluorescence staining of rat hippocampus shows strong positivity of the astrocytes.
Products for Astroglial Markers
AMAb90537
Target: AQP4
Clonality: Monoclonal
Validated Application: IHC*, WB
Sequence Identity Mouse/Rat: 93%/93%
HPA014784
Target: AQP4
Clonality: Polyclonal
Validated Application: IHC*, WB, ICC-IF
Sequence Identity Mouse/Rat: 93%/92%
HPA074762
Target: AQP9
Clonality: Polyclonal
Validated Application: IHC*
Sequence Identity Mouse/Rat: 55%/55%
HPA005785
Target: CD44
Clonality: Polyclonal
Validated Application: IHC*, WB*, ICC-IF
Sequence Identity Mouse/Rat: 51%/47%
AMAb91033
Target: GFAP
Clonality: Monoclonal
Validated Application: IHC*, WB*
Sequence Identity Mouse/Rat: 98%/100%
HPA056030
Target: GFAP
Clonality: Polyclonal
Validated Application: IHC*, WB, ICC-IF
Sequence Identity Mouse/Rat: 98%/100%
*Products with enhanced validation for the indicated application
Oligodendrocytes
Oligodendrocytes are glial cells within the CNS and spinal cord whose primary function is to form the insulating myelin sheaths around axons in the CNS. Furthermore, oligodendrocytes provide trophic support to neurons by producing different growth factors like BDNF and GDNF.
Product for Oligodendroglial Markers
HPA004947
Target: CD140/PDGFRA
Clonality: Polyclonal
Validated Application: ICC-IF
Sequence Identity Mouse/Rat: -
HPA004922
Target: CD133/PROML1A
Clonality: Polyclonal
Validated Application: IHC*
Sequence Identity Mouse/Rat: 57%/60%
HPA003254
Target: OLIG2
Clonality: Polyclonal
Validated Application: IHC*, WB
Sequence Identity Mouse/Rat: 93%/94%
HPA000842
Target: PROX1
Clonality: Polyclonal
Validated Application: ICC-IF
Sequence Identity Mouse/Rat: 100%/93%
HPA001030
Target: PROX1
Clonality: Polyclonal
Validated Application: ICC-IF
Sequence Identity Mouse/Rat: 100%/99%
AMAb91297
Target: SOX10
Clonality: Monoclonal
Validated Application: IHC, ICC-IF
Sequence Identity Mouse/Rat: 98%/98%
HPA058665
Target: SOX8
Clonality: Polyclonal
Validated Application: IHC*, ICC-IF
Sequence Identity Mouse/Rat: 73%/70%
*Products with enhanced validation for the indicated application
Products for Neuronal Stem Cell Markers
AMAb91406
Target: BRN2/POU3F2
Clonality: Monoclonal
Validated Applicstion: IHC, WB, ICC-IF
Sequence Identity Mouse/Rat: 100%/100%
HPA056261
Target: BRN2/POU3F2
Clonality: Polyclonal
Validated Application: ICC-IF
Sequence Identity Mouse/Rat: 100%/100%
HPA056402
Target: FGFR1
Clonality: Polyclonal
Validated Application: IHC. WB
Sequence Identity Mouse/Rat: 94%/96%
HPA050505
Target: FOXA2
Clonality: Polyclonal
Validated Application: IHC*, ICC-IF
Sequence Identity Mouse/Rat: 96%/82%
AMAb91375
Target: MAP2
Clonality: Monoclonal
Validated Application: IHC, WB, ICC-IF
Sequence Identity Mouse/Rat: 91%/90%
AMAb91375
Target: NEFL
Clonality: MOnoclonal
Validated Application: IHC, WB, ICC-IF
Sequence Identity Mouse/Rat: 97%/99%
*Products with enhanced validation for the indicated application
Related Publications
Jessell TM. et al. (2000) Principles of Neural Science, 4th Edition. New York: McGraw-Hill