Enhanced Validation

Monoclonal Anti-ZEB1 Antibody

zinc finger E-box binding homeobox 1
References (7)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human ZEB1
Alternative Gene Names
AREB6, BZP, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a
Open Datasheet
Price
$505.00
Product Number
AMAb90510
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human endometrium and liver tissues using AMAb90510 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ZEB1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[2 pictures]
Immunofluorescence staining in A549 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
zinc finger E-box binding homeobox 1
Target Gene
ZEB1
Belongs to Antibody Panel
Epithelial to Mesenchymal (EMT) Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000017863 (77%)

Mouse ENSMUSG00000024238 (74%)

Clonality
Monoclonal
Clone ID
CL0151
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90510 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90510 on the Human Protein Atlas
Protein Name
zinc finger E-box binding homeobox 1
Gene Name
ZEB1
Alternative Gene Names
AREB6, BZP, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a
UniProt ID
P37275
Gene (Ensembl)
ENSG00000148516
Entrez Gene ID
Entrez gene: 6935
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Parfenyev SE, Shabelnikov SV, Tolkunova EN, Barlev NA, Mittenberg AG

p53 Affects Zeb1 Interactome of Breast Cancer Stem Cells

Int J Mol Sci , 2023 Jun 6; 24(12):9806. Epub 2023 Jun 6

PubMed ID: 37372954 DOI: 10.3390/ijms24129806

Parfenyev SE, Shabelnikov SV, Pozdnyakov DY, Gnedina OO, Adonin LS, Barlev NA, Mittenberg AG

Proteomic Analysis of Zeb1 Interactome in Breast Carcinoma Cells

Molecules , 2021 May 24; 26(11):3143. Epub 2021 May 24

PubMed ID: 34074001 DOI: 10.3390/molecules26113143

Belulescu IC, Mărgăritescu C, Dumitrescu CI, Munteanu MC, Dăguci L, Mărgăritescu OC, Matei M

The immunophenotype of epithelial to mesenchymal transition inducing transcription factors in salivary gland adenoid cystic carcinomas

Rom J Morphol Embryol , 2020 Dec 30; 61(3):769-782. Epub 2020 Dec 30

PubMed ID: 33817718 DOI: 10.47162/RJME.61.3.15

Kinchen J, Chen HH, Parikh K, Antanaviciute A, Jagielowicz M, Fawkner-Corbett D, Ashley N, Cubitt L, Mellado-Gomez E, Attar M, Sharma E, Wills Q, Bowden R, Richter FC, Ahern D, Puri KD, Henault J, Gervais F, Koohy H, Simmons A

Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease

Cell , 2018 Oct 4; 175(2):372-386.e17

PubMed ID: 30270042 DOI: 10.1016/j.cell.2018.08.067

Roy A, Attarha S, Weishaupt H, Edqvist PH, Swartling FJ, Bergqvist M, Siebzehnrubl FA, Smits A, Pontén F, Tchougounova E

Serglycin as a potential biomarker for glioma: association of serglycin expression, extent of mast cell recruitment and glioblastoma progression

Oncotarget , 2017 Mar 1; 8(15):24815-24827. Epub 2017 Mar 1

PubMed ID: 28445977 DOI: 10.18632/oncotarget.15820

Müller S, Raulefs S, Bruns P, Afonso-Grunz F, Plötner A, Thermann R, Jäger C, Schlitter AM, Kong B, Regel I, Roth WK, Rotter B, Hoffmeier K, Kahl G, Koch I, Theis FJ, Kleeff J, Winter P, Michalski CW

Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer

Mol Cancer , 2015 Apr 25; 14:94. Epub 2015 Apr 25

PubMed ID: 25910082 DOI: 10.1186/s12943-015-0358-5

Varma S, Mahavadi P, Sasikumar S, Cushing L, Hyland T, Rosser AE, Riccardi D, Lu J, Kalin TV, Kalinichenko VV, Guenther A, Ramirez MI, Pardo A, Selman M, Warburton D

Grainyhead-like 2 (GRHL2) distribution reveals novel pathophysiological differences between human idiopathic pulmonary fibrosis and mouse models of pulmonary fibrosis

Am J Physiol Lung Cell Mol Physiol , 2014 Mar 1; 306(5):L405-L419. Epub 2013 Dec 27

PubMed ID: 24375798 DOI: 10.1152/ajplung.00143.2013

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ZEB1 (HPA027524)
Corresponding Antigens
PrEST Antigen ZEB1 (APrEST84772)

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