Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000018087 (99%)
Mouse ENSMUSG00000026728 (99%)
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Contribution from Undisclosed customer — April 26, 2018
Western blot using Anti-VIM antibody (AMAb90516) in K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.
Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.
PVDF membrane was probed with 0.7 ug/mL of Anti-VIM Antibody (AMAb90516). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in
the parental K562 cell line whereas in the knockout K562 cell line no band was detected.
The experiment was conducted under reducing conditions.
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.