Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000026728 (99%)
Rat ENSRNOG00000018087 (99%)
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Contribution from Undisclosed customer — April 26, 2018
Western blot using Anti-VIM antibody (AMAb90516) in K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.
Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line.
PVDF membrane was probed with 0.7 ug/mL of Anti-VIM Antibody (AMAb90516). A specific band was detected for Vimentin at approximately 55 kDa (as indicated) in
the parental K562 cell line whereas in the knockout K562 cell line no band was detected.
The experiment was conducted under reducing conditions.
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