Enhanced Validation

Monoclonal Anti-TUFM Antibody

Tu translation elongation factor, mitochondrial
References (3)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human TUFM
Alternative Gene Names
EF-TuMT, EFTu
Open Datasheet
Price
$505.00
Product Number
AMAb90964
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.
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[3 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TUFM antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Tu translation elongation factor, mitochondrial
Target Gene
TUFM
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000018604 (96%)

Mouse ENSMUSG00000030735 (95%)

Clonality
Monoclonal
Clone ID
CL2242
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90964 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90964 on the Human Protein Atlas
Protein Name
Tu translation elongation factor, mitochondrial
Gene Name
TUFM
Alternative Gene Names
EF-TuMT, EFTu
UniProt ID
P49411
Gene (Ensembl)
ENSG00000178952
Entrez Gene ID
Entrez gene: 7284
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Banfi C, Baetta R, Barbieri SS, Brioschi M, Guarino A, Ghilardi S, Sandrini L, Eligini S, Polvani G, Bergman O, Eriksson P, Tremoli E

Prenylcysteine oxidase 1, an emerging player in atherosclerosis

Commun Biol , 2021 Sep 21; 4:1109. Epub 2021 Sep 21

PubMed ID: 34548610 DOI: 10.1038/s42003-021-02630-z

Kannangara AR, Poole DM, McEwan CM, Youngs JC, Weerasekara VK, Thornock AM, Lazaro MT, Balasooriya ER, Oh LM, Soderblom EJ, Lee JJ, Simmons DL, Andersen JL

BioID reveals an ATG9A interaction with ATG13‐ATG101 in the degradation of p62/SQSTM1‐ubiquitin clusters

EMBO Rep , 2021 Aug 9; 22(10):e51136. Epub 2021 Aug 9

PubMed ID: 34369648 DOI: 10.15252/embr.202051136

Zottel A, Jovčevska I, Šamec N, Mlakar J, Šribar J, Križaj I, Skoblar Vidmar M, Komel R

Anti-vimentin, anti-TUFM, anti-NAP1L1 and anti-DPYSL2 nanobodies display cytotoxic effect and reduce glioblastoma cell migration

Ther Adv Med Oncol , 2020 Apr 28; 12:1758835920915302. Epub 2020 Apr 28

PubMed ID: 32426045 DOI: 10.1177/1758835920915302

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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