Monoclonal Anti-TUBB3 Antibody

Tubulin beta 3 class iii
References (1)
Recommended Applications
Product Description
Monoclonal Antibody against Human TUBB3
Alternative Gene Names
beta-4, CFEOM3, CFEOM3A, FEOM3
Open Datasheet
Price
$505.00
Product Number
AMAb91394
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[6 pictures]
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.
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[2 pictures]
Western blot analysis in human cell line SH-SY5Y.
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[2 pictures]
Immunofluorescence staining of A549 cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Tubulin beta 3 class iii
Target Gene
TUBB3
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000062380 (93%)

Rat ENSRNOG00000017209 (86%)

Clonality
Monoclonal
Clone ID
CL5813
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50000 - 1:100000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91394 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91394 on the Human Protein Atlas
Protein Name
Tubulin beta 3 class iii
Gene Name
TUBB3
Alternative Gene Names
beta-4, CFEOM3, CFEOM3A, FEOM3
UniProt ID
Q13509
Gene (Ensembl)
ENSG00000258947
Entrez Gene ID
Entrez gene: 10381
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Junaković A, Kopić J, Duque A, Rakic P, Krsnik Ž, Kostović I

Laminar dynamics of deep projection neurons and mode of subplate formation are hallmarks of histogenetic subdivisions of the human cingulate cortex before onset of arealization

Brain Struct Funct , 2023 Jan 2; 228(2):613-633. Epub 2023 Jan 2

PubMed ID: 36592215 DOI: 10.1007/s00429-022-02606-7

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-TUBB3 (AMAb91395)

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