Enhanced Validation

Monoclonal Anti-TET2 Antibody

Tet methylcytosine dioxygenase 2
References (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human TET2
Alternative Gene Names
FLJ20032, KIAA1546
Open Datasheet
Price
$505.00
Product Number
AMAb91439
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human placenta and pancreas tissues using AMAb91439 antibody. Corresponding TET2 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in human cell line HL-60.
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[1 pictures]
Immunofluorescence staining of U-2 OS cells using the Anti-TET2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Tet methylcytosine dioxygenase 2
Target Gene
TET2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000040943 (89%)

Rat ENSRNOG00000023579 (84%)

Clonality
Monoclonal
Clone ID
CL6873
Isotype
IgG2b
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91439 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91439 on the Human Protein Atlas
Protein Name
Tet methylcytosine dioxygenase 2
Gene Name
TET2
Alternative Gene Names
FLJ20032, KIAA1546
UniProt ID
Q6N021
Gene (Ensembl)
ENSG00000168769
Entrez Gene ID
Entrez gene: 54790
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Fujikura K, Alruwaii ZI, Haffner MC, Trujillo MA, Roberts NJ, Hong SM, Macgregor-Das A, Goggins MG, Roy S, Meeker AK, Ding D, Wright M, He J, Hruban RH, Wood LD

Downregulation of 5-hydroxymethylcytosine is an early event in pancreatic tumorigenesis

J Pathol , 2021 May 21; 254(3):279-288. Epub 2021 May 21

PubMed ID: 33870509 DOI: 10.1002/path.5682

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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