Enhanced Validation

Monoclonal Anti-SOX9 Antibody

SRY (sex determining region Y)-box 9
References (8)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human SOX9
Alternative Gene Names
CMD1, CMPD1, SRA1
Open Datasheet
Price
$505.00
Product Number
AMAb90795
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[7 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb90795 antibody. Corresponding SOX9 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX9 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of U-251 cells using the anti-SOX9 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
SRY (sex determining region Y)-box 9
Target Gene
SOX9
Verified Species Reactivity
Human, Mouse
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000000567 (97%)

Rat ENSRNOG00000002607 (96%)

Clonality
Monoclonal
Clone ID
CL0639
Isotype
IgG2a
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90795 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90795 on the Human Protein Atlas
Protein Name
SRY (sex determining region Y)-box 9
Gene Name
SOX9
Alternative Gene Names
CMD1, CMPD1, SRA1
UniProt ID
P48436
Gene (Ensembl)
ENSG00000125398
Entrez Gene ID
Entrez gene: 6662
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Chen M, Li S, Liu S, Zhang Y, Cui X, Lv L, Liu B, Zheng A, Wang Q, Duo S, Gao F

Infection of SARS-CoV-2 causes severe pathological changes in mouse testis

J Genet Genomics , . Epub 2022 Dec 6

PubMed ID: 36494057 DOI: 10.1016/j.jgg.2022.11.011

Nimmakayala RK, Ogunleye AO, Parte S, Krishna Kumar N, Raut P, Varadharaj V, Perumal NK, Nallasamy P, Rauth S, Cox JL, Lele SM, Batra SK, Ponnusamy MP

PAF1 cooperates with YAP1 in metaplastic ducts to promote pancreatic cancer

Cell Death Dis , 2022 Oct 1; 13(10):839. Epub 2022 Oct 1

PubMed ID: 36180487 DOI: 10.1038/s41419-022-05258-x

Corell A, Gómez Vecchio T, Ferreyra Vega S, Dénes A, Neimantaite A, Hagerius A, Barchéus H, Solheim O, Lindskog C, Bontell TO, Carén H, Jakola AS, Smits A

Stemness and clinical features in relation to the subventricular zone in diffuse lower-grade glioma: an exploratory study

Neurooncol Adv , 2022 Jun 4; 4(1):vdac074. Epub 2022 Jun 4

PubMed ID: 35795469 DOI: 10.1093/noajnl/vdac074

Lam KK, Tang CL, Tan E, Wong SH, Cheah PY

KRAS mutation‐independent downregulation of MAPK/PI3K signaling in colorectal cancer

Mol Oncol , 2022 Jan 1; 16(5):1171-1183. Epub 2022 Jan 1

PubMed ID: 34919787 DOI: 10.1002/1878-0261.13163

Mascadri F, Bolognesi MM, Pilla D, Cattoretti G

Rejuvenated Vintage Tissue Sections Highlight Individual Antigen Fate During Processing and Long-term Storage

J Histochem Cytochem , 2021 Sep 19; 69(10):659-667. Epub 2021 Sep 19

PubMed ID: 34541944 DOI: 10.1369/00221554211047287

Seime T, Akbulut AC, Liljeqvist ML, Siika A, Jin H, Winski G, van Gorp RH, Karlöf E, Lengquist M, Buckler AJ, Kronqvist M, Waring OJ, Lindeman JH, Biessen EA, Maegdefessel L, Razuvaev A, Schurgers LJ, Hedin U, Matic L

Proteoglycan 4 Modulates Osteogenic Smooth Muscle Cell Differentiation during Vascular Remodeling and Intimal Calcification

Cells , 2021 May 21; 10(6):1276. Epub 2021 May 21

PubMed ID: 34063989 DOI: 10.3390/cells10061276

Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K

Generation of orthotopically functional salivary gland from embryonic stem cells

Nat Commun , 2018 Oct 11; 9:4216. Epub 2018 Oct 11

PubMed ID: 30310071 DOI: 10.1038/s41467-018-06469-7

Chen W, Zhao W, Zhang L, Wang L, Wang J, Wan Z, Hong Y, Yu L

MALAT1-miR-101-SOX9 feedback loop modulates the chemo-resistance of lung cancer cell to DDP via Wnt signaling pathway

Oncotarget , 2017 Oct 9; 8(55):94317-94329. Epub 2017 Oct 9

PubMed ID: 29212230 DOI: 10.18632/oncotarget.21693

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-SOX9 (HPA001758)
Corresponding Antigens
PrEST Antigen SOX9 (APrEST84775)

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