Enhanced Validation

Anti-SOX9 Antibody

SRY (sex determining region Y)-box 9
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human SOX9
Alternative Gene Names
CMD1, CMPD1, SRA1
Open Datasheet
Price
$459.00
Product Number
AMAb90795
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[7 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb90795 antibody. Corresponding SOX9 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX9 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of U-251 cells using the anti-SOX9 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
SRY (sex determining region Y)-box 9
Target Gene
SOX9
Verified Species Reactivity
Human, Mouse
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000000567 (97%)

Rat ENSRNOG00000002607 (96%)

Clonality
Monoclonal
Clone ID
CL0639
Isotype
IgG2a
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90795 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90795 on the Human Protein Atlas
Protein Name
SRY (sex determining region Y)-box 9
Gene Name
SOX9
Alternative Gene Names
CMD1, CMPD1, SRA1
UniProt ID
P48436
Gene (Ensembl)
ENSG00000125398
Entrez Gene ID
Entrez gene: 6662
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K

Generation of orthotopically functional salivary gland from embryonic stem cells

Nat Commun , 2018 Oct 11; 9:4216. Epub 2018 Oct 11

PubMed ID: 30310071 DOI: 10.1038/s41467-018-06469-7

Chen W, Zhao W, Zhang L, Wang L, Wang J, Wan Z, Hong Y, Yu L

MALAT1-miR-101-SOX9 feedback loop modulates the chemo-resistance of lung cancer cell to DDP via Wnt signaling pathway

Oncotarget , 2017 Oct 9; 8(55):94317-94329. Epub 2017 Oct 9

PubMed ID: 29212230 DOI: 10.18632/oncotarget.21693

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Alternative Antibodies
Anti-SOX9 (HPA001758)
Corresponding Antigens
PrEST Antigen SOX9 (APrEST84775)

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