Enhanced Validation

Anti-SATB2 Antibody

SATB homeobox 2
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human SATB2
Alternative Gene Names
FLJ21474, KIAA1034
Open Datasheet
Price
$450.00
Product Number
AMAb90679
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[7 pictures]
Immunohistochemistry analysis in human rectum and liver tissues using AMAb90679 antibody. Corresponding SATB2 RNA-seq data are presented for the same tissues.
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[2 pictures]
Lane 1: Marker [kDa]
Lane 2: Human cell line HEL
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[1 pictures]
Immunofluorescence staining of U-138 MG cells using the Anti-SATB2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
SATB homeobox 2
Target Gene
SATB2
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
KECPLSQSMISSIVNSTYYANVSATKCQEFGRWYKKYKKIKVERVERENLSDYCVLGQRPMHLPNMNQLASLGKTNEQSPHSQIHHSTPIRNQVPALQPIMSPGLLSPQLSPQLV
Epitope
Binds to an epitope located within the peptide sequence SLGKTNEQSPHSQIH as determined by overlapping synthetic peptides.
Verified Species Reactivity
Human, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000010188 (100%)

Mouse ENSMUSG00000038331 (100%)

Clonality
Monoclonal
Clone ID
CL0320
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90679 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90679 on the Human Protein Atlas
Protein Name
SATB homeobox 2
Gene Name
SATB2
Alternative Gene Names
FLJ21474, KIAA1034
UniProt ID
Q9UPW6
Gene (Ensembl)
ENSG00000119042
Entrez Gene ID
Entrez gene: 23314
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Hedner C, Gaber A, Korkocic D, Nodin B, Uhlén M, Kuteeva E, Johannesson H, Jirström K, Eberhard J

SATB1 is an independent prognostic factor in radically resected upper gastrointestinal tract adenocarcinoma

Virchows Arch , 2014 Dec; 465(6):649-659. Epub 2014 Oct 19

PubMed ID: 25326863 DOI: 10.1007/s00428-014-1667-6

Elebro J, Heby M, Gaber A, Nodin B, Jonsson L, Fristedt R, Uhlén M, Jirström K, Eberhard J

Prognostic and treatment predictive significance of SATB1 and SATB2 expression in pancreatic and periampullary adenocarcinoma

J Transl Med , 2014 Oct 17; 12:289. Epub 2014 Oct 17

PubMed ID: 25323550 DOI: 10.1186/s12967-014-0289-8

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