Enhanced Validation

Monoclonal Anti-SATB2 Antibody

SATB homeobox 2
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human SATB2
Alternative Gene Names
FLJ21474, KIAA1034
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
SATB homeobox 2
Target Gene
Verified Species Reactivity
Human, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000038331 (100%)

Rat ENSRNOG00000010188 (100%)

Clone ID
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
SATB homeobox 2
Gene Name
Alternative Gene Names
FLJ21474, KIAA1034
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature.
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Siesing C, Petersson A, Ulfarsdottir T, Chattopadhyay S, Nodin B, Eberhard J, Brändstedt J, Syk I, Gisselsson D, Jirström K

Delineating the intra-patient heterogeneity of molecular alterations in treatment-naïve colorectal cancer with peritoneal carcinomatosis

Mod Pathol , 2022 Feb 15; 35(7):979-988. Epub 2022 Feb 15

PubMed ID: 35169225 DOI: 10.1038/s41379-022-01012-y

Hedner C, Gaber A, Korkocic D, Nodin B, Uhlén M, Kuteeva E, Johannesson H, Jirström K, Eberhard J

SATB1 is an independent prognostic factor in radically resected upper gastrointestinal tract adenocarcinoma

Virchows Arch , 2014 Dec; 465(6):649-659. Epub 2014 Oct 19

PubMed ID: 25326863 DOI: 10.1007/s00428-014-1667-6

Elebro J, Heby M, Gaber A, Nodin B, Jonsson L, Fristedt R, Uhlén M, Jirström K, Eberhard J

Prognostic and treatment predictive significance of SATB1 and SATB2 expression in pancreatic and periampullary adenocarcinoma

J Transl Med , 2014 Oct 17; 12:289. Epub 2014 Oct 17

PubMed ID: 25323550 DOI: 10.1186/s12967-014-0289-8

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