Enhanced Validation

Anti-S100B Antibody

S100 calcium binding protein B
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human S100B
Alternative Gene Names
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
S100 calcium binding protein B
Target Gene
Belongs to Antibody Panel
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000033208 (99%)

Rat ENSRNOG00000001295 (98%)

Clone ID
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
S100 calcium binding protein B
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Emre C, Do KV, Jun B, Hjorth E, Alcalde SG, Kautzmann MA, Gordon WC, Nilsson P, Bazan NG, Schultzberg M

Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer’s disease

Acta Neuropathol Commun , 2021 Jun 29; 9:116. Epub 2021 Jun 29

PubMed ID: 0 DOI: 10.1186/s40478-021-01216-4

Liao MF, Yeh SR, Lu KT, Hsu JL, Chao PK, Hsu HC, Peng CH, Lee YL, Hung YH, Ro LS

Interactions between Autophagy, Proinflammatory Cytokines, and Apoptosis in Neuropathic Pain: Granulocyte Colony Stimulating Factor as a Multipotent Therapy in Rats with Chronic Constriction Injury

Biomedicines , 2021 May 12; 9(5):542. Epub 2021 May 12

PubMed ID: 34066206 DOI: 10.3390/biomedicines9050542

Kano M, Takanashi M, Oyama G, Yoritaka A, Hatano T, Shiba-Fukushima K, Nagai M, Nishiyama K, Hasegawa K, Inoshita T, Ishikawa KI, Akamatsu W, Imai Y, Bolognin S, Schwamborn JC, Hattori N

Reduced astrocytic reactivity in human brains and midbrain organoids with PRKN mutations

NPJ Parkinsons Dis , 2020 Nov 13; 6:33. Epub 2020 Nov 13

PubMed ID: 33298969 DOI: 10.1038/s41531-020-00137-8

Martin E, Aigrot MS, Grenningloh R, Stankoff B, Lubetzki C, Boschert U, Zalc B

Bruton’s Tyrosine Kinase Inhibition Promotes Myelin Repair

Brain Plast , 2020 Oct 1; 5(2):123-133. Epub 2020 Oct 1

PubMed ID: 33282676 DOI: 10.3233/BPL-200100

Lu Y, Sareddy GR, Wang J, Zhang Q, Tang FL, Pratap UP, Tekmal RR, Vadlamudi RK, Brann DW

Neuron-Derived Estrogen Is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain

J Neurosci , 2020 Sep 16; 40(38):7355-7374

PubMed ID: 32817249 DOI: 10.1523/JNEUROSCI.0115-20.2020

Habela CW, Yoon KJ, Kim NS, Taga A, Bell K, Bergles DE, Maragakis NJ, Ming GL, Song H

Persistent Cyfip1 Expression Is Required to Maintain the Adult Subventricular Zone Neurogenic Niche

J Neurosci , 2020 Mar 4; 40(10):2015-2024

PubMed ID: 31988061 DOI: 10.1523/JNEUROSCI.2249-19.2020

Wu J, Tian W, Liu Y, Wang HJ, Zheng J, Wang X, Pan H, Li J, Luo J, Yang X, Lau LF, Ghashghaei HT, Shen Q

Ependyma‐expressed CCN1 restricts the size of the neural stem cell pool in the adult ventricular‐subventricular zone

EMBO J , 2020 Feb 3; 39(5):e101679. Epub 2020 Feb 3

PubMed ID: 32009252 DOI: 10.15252/embj.2019101679

Barca‐Mayo O, Boender AJ, Armirotti A, De Pietri Tonelli D

Deletion of astrocytic BMAL1 results in metabolic imbalance and shorter lifespan in mice

Glia , 2019 Dec 13; 68(6):1131-1147. Epub 2019 Dec 13

PubMed ID: 31833591 DOI: 10.1002/glia.23764

Allard DE, Wang Y, Li JJ, Conley B, Xu EW, Sailer D, Kimpston C, Notini R, Smith CJ, Koseoglu E, Starmer J, Zeng XL, Howard JF Jr, Hoke A, Scherer SS, Su MA

Schwann cell–derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment

J Clin Invest , 2018 Sep 17; 128(10):4727-4741. Epub 2018 Sep 17

PubMed ID: 30222134 DOI: 10.1172/JCI99308

Sharma S, Khadimallah I, Corya AW, Ali YO, Rao X, Liu Y, Lu HC

Presymptomatic change in microRNAs modulates Tau pathology

Sci Rep , 2018 Jun 18; 8:9251. Epub 2018 Jun 18

PubMed ID: 29915328 DOI: 10.1038/s41598-018-27527-6

Lin YT, Seo J, Gao F, Feldman HM, Wen HL, Penney J, Cam HP, Gjoneska E, Raja WK, Cheng J, Rueda R, Kritskiy O, Abdurrob F, Peng Z, Milo B, Yu CJ, Elmsaouri S, Dey D, Ko T, Yankner BA, Tsai LH

APOE4 causes widespread molecular and cellular alterations associated with Alzheimer’s disease phenotypes in human iPSC-derived brain cell types

Neuron , 2018 May 31; 98(6):1141-1154.e7. Epub 2018 May 31

PubMed ID: 29861287 DOI: 10.1016/j.neuron.2018.05.008

Cao J, Lin Y, Han Y, Ding S, Fan Y, Pan Y, Zhao B, Guo Q, Sun W, Wan J, Tong X

Expression of nerve growth factor carried by pseudotyped lentivirus improves neuron survival and cognitive functional recovery of post‐ischemia in rats

CNS Neurosci Ther , 2018 Feb 6; 24(6):508-518. Epub 2018 Feb 6

PubMed ID: 29409115 DOI: 10.1111/cns.12818

Tripathi P, Rodriguez-Muela N, Klim JR, de Boer AS, Agrawal S, Sandoe J, Lopes CS, Ogliari KS, Williams LA, Shear M, Rubin LL, Eggan K, Zhou Q

Reactive Astrocytes Promote ALS-like Degeneration and Intracellular Protein Aggregation in Human Motor Neurons by Disrupting Autophagy through TGF-β1

Stem Cell Reports , 2017 Jul 14; 9(2):667-680. Epub 2017 Jul 14

PubMed ID: 28712846 DOI: 10.1016/j.stemcr.2017.06.008

Barca-Mayo O, Pons-Espinal M, Follert P, Armirotti A, Berdondini L, De Pietri Tonelli D

Astrocyte deletion of Bmal1 alters daily locomotor activity and cognitive functions via GABA signalling

Nat Commun , 2017 Feb 10; 8:14336. Epub 2017 Feb 10

PubMed ID: 28186121 DOI: 10.1038/ncomms14336

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