Enhanced Validation

Monoclonal Anti-S100A4 Antibody

S100 calcium binding protein A4
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human S100A4
Alternative Gene Names
18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Open Datasheet
Price
$505.00
Product Number
AMAb90599
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human tonsil and pancreas tissues using AMAb90599 antibody. Corresponding S100A4 RNA-seq data are presented for the same tissues.
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[1 pictures]
Lane 1: Marker [kDa]
Lane 2: Human tonsil tissue lysate
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[1 pictures]
Immunofluorescence staining of BJ cells using the anti-S100A4 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
S100 calcium binding protein A4
Target Gene
S100A4
Belongs to Antibody Panel
Epithelial to Mesenchymal (EMT) Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000001020 (93%)

Rat ENSRNOG00000011821 (91%)

Clonality
Monoclonal
Clone ID
CL0240
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:2500 - 1:5000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90599 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90599 on the Human Protein Atlas
Protein Name
S100 calcium binding protein A4
Gene Name
S100A4
Alternative Gene Names
18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
UniProt ID
P26447
Gene (Ensembl)
ENSG00000196154
Entrez Gene ID
Entrez gene: 6275
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Athanasiou AT, Nussbaumer T, Kummer S, Hofer M, Johnston IG, Staltner M, Allmer DM, Scott MC, Vogl C, Fenger JM, Modiano JF, Walter I, Steinborn R

S100A4 mRNA-protein relationship uncovered by measurement noise reduction

J Mol Med (Berl) , 2020 Apr 15; 98(5):735-749. Epub 2020 Apr 15

PubMed ID: 32296879 DOI: 10.1007/s00109-020-01898-8

Larsson K, Kock A, Idborg H, Arsenian Henriksson M, Martinsson T, Johnsen JI, Korotkova M, Kogner P, Jakobsson PJ

COX/mPGES-1/PGE2 pathway depicts an inflammatory-dependent high-risk neuroblastoma subset

Proc Natl Acad Sci U S A , 2015 Jun 30; 112(26):8070-8075. Epub 2015 Jun 15

PubMed ID: 26080408 DOI: 10.1073/pnas.1424355112

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