Lane 2: Human tonsil tissue lysate
Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000001020 (93%)
Rat ENSRNOG00000011821 (91%)
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
COX/mPGES-1/PGE2 pathway depicts an inflammatory-dependent high-risk neuroblastoma subset
Proc Natl Acad Sci U S A , 2015 Jun 30; 112(26):8070-8075. Epub 2015 Jun 15
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