Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: S100A4 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418991)
Human Protein Atlas
Dilution: 1:2500 - 1:5000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
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