Enhanced Validation

Monoclonal Anti-RUNX2 Antibody

runt-related transcription factor 2
References (2)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human RUNX2
Alternative Gene Names
AML3, CBFA1, CCD, CCD1, PEBP2A1, PEBP2aA1
Open Datasheet
Price
$505.00
Product Number
AMAb90591
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells outside the reaction centrum.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[1 pictures]
Immunofluorescence staining of RH-30 cells using the Anti-RUNX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
runt-related transcription factor 2
Target Gene
RUNX2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000039153 (100%)

Rat ENSRNOG00000020193 (81%)

Clonality
Monoclonal
Clone ID
CL0232
Isotype
IgG2a
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90591 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90591 on the Human Protein Atlas
Protein Name
runt-related transcription factor 2
Gene Name
RUNX2
Alternative Gene Names
AML3, CBFA1, CCD, CCD1, PEBP2A1, PEBP2aA1
UniProt ID
Q13950
Gene (Ensembl)
ENSG00000124813
Entrez Gene ID
Entrez gene: 860
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Seime T, Akbulut AC, Liljeqvist ML, Siika A, Jin H, Winski G, van Gorp RH, Karlöf E, Lengquist M, Buckler AJ, Kronqvist M, Waring OJ, Lindeman JH, Biessen EA, Maegdefessel L, Razuvaev A, Schurgers LJ, Hedin U, Matic L

Proteoglycan 4 Modulates Osteogenic Smooth Muscle Cell Differentiation during Vascular Remodeling and Intimal Calcification

Cells , 2021 May 21; 10(6):1276. Epub 2021 May 21

PubMed ID: 34063989 DOI: 10.3390/cells10061276

Ibarrola J, Martínez-Martínez E, Sádaba JR, Arrieta V, García-Peña A, Álvarez V, Fernández-Celis A, Gainza A, Rossignol P, Cachofeiro Ramos V, López-Andrés N

Beneficial Effects of Galectin-3 Blockade in Vascular and Aortic Valve Alterations in an Experimental Pressure Overload Model

Int J Mol Sci , 2017 Jul 31; 18(8):1664. Epub 2017 Jul 31

PubMed ID: 28758988 DOI: 10.3390/ijms18081664

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-RUNX2 (AMAb90594)
Anti-RUNX2 (HPA022040)
Corresponding Antigens
PrEST Antigen RUNX2 (APrEST86701)

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