Monoclonal Anti-PRAME Antibody

preferentially expressed antigen in melanoma
Recommended Applications
Product Description
Monoclonal Antibody against Human PRAME
Alternative Gene Names
CT130, MAPE
Open Datasheet
Price
$505.00
Product Number
AMAb91329
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[1 pictures]
Western blot analysis in human cell line U-2 OS.
Show all images
[1 pictures]
Immunofluorescence staining of U-2 OS cells using the Anti-PRAME monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
preferentially expressed antigen in melanoma
Target Gene
PRAME
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000028591 (55%)

Rat ENSRNOG00000037035 (55%)

Clonality
Monoclonal
Clone ID
CL5146
Isotype
IgG2a
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91329 on the Human Protein Atlas
Protein Name
preferentially expressed antigen in melanoma
Gene Name
PRAME
Alternative Gene Names
CT130, MAPE
UniProt ID
P78395
Gene (Ensembl)
ENSG00000185686
Entrez Gene ID
Entrez gene: 23532
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Did we miss your publication?

Have you published using AMAb91329? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-PRAME (AMAb91330)
Anti-PRAME (HPA045153)
Anti-PRAME (HPA068562)
Corresponding Antigens
PrEST Antigen PRAME (APrEST70289)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch