Enhanced Validation

Anti-PODXL Antibody

podocalyxin-like
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human PODXL
Alternative Gene Names
Gp200, PC, PCLP
Price
$437.00
Product Number
AMAb90667
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
podocalyxin-like
Target gene
PODXL
Antigen sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
LPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQTQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLPAKDVYERLKDKWDELKEAGVSDMKLGD
Epitope
Binds to an epitope located within the peptide sequence IHTKLPAKDVYERLK as determined by overlapping synthetic peptides.
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000025608 (43%)

Rat ENSRNOG00000012495 (41%)

Clonality
Monoclonal
Clone ID
CL0308
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
podocalyxin-like
Gene Name
PODXL
Alternative Gene Names
Gp200, PC, PCLP
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Boman K, Andersson G, Wennersten C, Nodin B, Ahlgren G, Jirström K

Podocalyxin-like and RNA-binding motif protein 3 are prognostic biomarkers in urothelial bladder cancer: a validatory study

Biomark Res , 2017 Mar 14; 5:10. Epub 2017 Mar 14

PubMed ID: 28293425 DOI: 10.1186/s40364-017-0090-y

Boman K, Larsson AH, Segersten U, Kuteeva E, Johannesson H, Nodin B, Eberhard J, Uhlén M, Malmström PU, Jirström K

Membranous expression of podocalyxin-like protein is an independent factor of poor prognosis in urothelial bladder cancer

Br J Cancer , 2013 Jun 11; 108(11):2321-2328. Epub 2013 May 7

PubMed ID: 23652315 DOI: 10.1038/bjc.2013.215

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