Enhanced Validation

Monoclonal Anti-PLA2R1 Antibody

phospholipase A2 receptor 1, 180kDa
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human PLA2R1
Alternative Gene Names
CLEC13C, PLA2-R, PLA2G1R, PLA2IR
Open Datasheet
Price
$505.00
Product Number
AMAb90772
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[4 pictures]
Immunohistochemistry analysis in human kidney and pancreas tissues using AMAb90772 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Western blot analysis in human kidney tissue.
Show all images
[2 pictures]
Immunofluorescence staining of HeLa cells using the Anti-PLA2R1 monoclonal antibody, showing specific staining in the cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
phospholipase A2 receptor 1, 180kDa
Target Gene
PLA2R1
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000008129 (76%)

Mouse ENSMUSG00000054580 (74%)

Clonality
Monoclonal
Clone ID
CL0474
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20000 - 1:50000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90772 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90772 on the Human Protein Atlas
Protein Name
phospholipase A2 receptor 1, 180kDa
Gene Name
PLA2R1
Alternative Gene Names
CLEC13C, PLA2-R, PLA2G1R, PLA2IR
UniProt ID
Q13018
Gene (Ensembl)
ENSG00000153246
Entrez Gene ID
Entrez gene: 22925
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Guo S, Kapp ME, Beltran DM, Cardona CY, Caster DJ, Reichel RR, Fogo AB

Spectrum of Kidney Diseases in Patients With Hepatitis C Virus Infection: A 10-Year Study

Am J Clin Pathol , 2021 Feb 24; 156(3):399-408. Epub 2021 Feb 24

PubMed ID: 33623996 DOI: 10.1093/ajcp/aqaa238

Griveau A, Wiel C, Le Calvé B, Ziegler DV, Djebali S, Warnier M, Martin N, Marvel J, Vindrieux D, Bergo MO, Bernard D

Targeting the phospholipase A2 receptor ameliorates premature aging phenotypes

Aging Cell , 2018 Sep 14; 17(6):e12835. Epub 2018 Sep 14

PubMed ID: 30216637 DOI: 10.1111/acel.12835

Did we miss your publication?

Have you published using AMAb90772? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-PLA2R1 (AMAb90775)
Anti-PLA2R1 (HPA012657)
Corresponding Antigens
PrEST Antigen PLA2R1 (APrEST72110)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch