Enhanced Validation

Monoclonal Anti-PBRM1 Antibody

polybromo 1
References (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human PBRM1
Alternative Gene Names
BAF180, PB1
Open Datasheet
Price
$505.00
Product Number
AMAb90690
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human endometrium and skeletal muscle tissues using AMAb90690 antibody. Corresponding PBRM1 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PBRM1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
polybromo 1
Target Gene
PBRM1
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000042323 (97%)

Rat ENSRNOG00000028227 (96%)

Clonality
Monoclonal
Clone ID
CL0331
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90690 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
polybromo 1
Gene Name
PBRM1
Alternative Gene Names
BAF180, PB1
UniProt ID
Q86U86
Gene (Ensembl)
ENSG00000163939
Entrez Gene ID
Entrez gene: 55193
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Mota ST, Vecchi L, Zóia MA, Oliveira FM, Alves DA, Dornelas BC, Bezerra SM, Andrade VP, Maia YC, Neves AF, Goulart LR, Araújo TG

New Insights into the Role of Polybromo-1 in Prostate Cancer

Int J Mol Sci , 2019 Jun 12; 20(12):2852. Epub 2019 Jun 12

PubMed ID: 31212728 DOI: 10.3390/ijms20122852

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-PBRM1 (HPA015629)
Anti-PBRM1 (HPA059373)
Corresponding Antigens
PrEST Antigen PBRM1 (APrEST72688)

Questions?

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