Enhanced Validation

Monoclonal Anti-P4HA2 Antibody

prolyl 4-hydroxylase, alpha polypeptide II
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human P4HA2
Alternative Gene Names
C-P4Halpha(II)
Open Datasheet
Price
$505.00
Product Number
AMAb90710
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human epididymis and skeletal muscle tissues using AMAb90710 antibody. Corresponding P4HA2 RNA-seq data are presented for the same tissues.
Show all images
[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
prolyl 4-hydroxylase, alpha polypeptide II
Target Gene
P4HA2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000033663 (91%)

Mouse ENSMUSG00000018906 (90%)

Clonality
Monoclonal
Clone ID
CL0351
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90710 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
prolyl 4-hydroxylase, alpha polypeptide II
Gene Name
P4HA2
Alternative Gene Names
C-P4Halpha(II)
UniProt ID
O15460
Gene (Ensembl)
ENSG00000072682
Entrez Gene ID
Entrez gene: 8974
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-P4HA2 (HPA016997)
Anti-P4HA2 (HPA027824)
Corresponding Antigens
PrEST Antigen P4HA2 (APrEST71291)

Questions?

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