Enhanced Validation

Monoclonal Anti-NOP56 Antibody

NOP56 ribonucleoprotein homolog (yeast)
References (2)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human NOP56
Alternative Gene Names
NOL5A, SCA36
Open Datasheet
Price
$505.00
Product Number
AMAb91013
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemical staining of human prostate cancer shows strong nucleolar positivity in glandular cells.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NOP56 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-NOP56 monoclonal antibody, showing nucleoli-specific staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
NOP56 ribonucleoprotein homolog (yeast)
Target Gene
NOP56
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000007128 (62%)

Mouse ENSMUSG00000027405 (59%)

Clonality
Monoclonal
Clone ID
CL2603
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91013 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91013 on the Human Protein Atlas
Protein Name
NOP56 ribonucleoprotein homolog (yeast)
Gene Name
NOP56
Alternative Gene Names
NOL5A, SCA36
UniProt ID
O00567
Gene (Ensembl)
ENSG00000101361
Entrez Gene ID
Entrez gene: 100302138
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Banfi C, Baetta R, Barbieri SS, Brioschi M, Guarino A, Ghilardi S, Sandrini L, Eligini S, Polvani G, Bergman O, Eriksson P, Tremoli E

Prenylcysteine oxidase 1, an emerging player in atherosclerosis

Commun Biol , 2021 Sep 21; 4:1109. Epub 2021 Sep 21

PubMed ID: 34548610 DOI: 10.1038/s42003-021-02630-z

Vellky JE, Ricke EA, Huang W, Ricke WA

Expression, Localization, and Function of the Nucleolar Protein BOP1 in Prostate Cancer Progression

Am J Pathol , 2021 Jan; 191(1):168-179

PubMed ID: 33039351 DOI: 10.1016/j.ajpath.2020.09.010

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-NOP56 (HPA049918)
Corresponding Antigens
PrEST Antigen NOP56 (APrEST85008)

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