Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Human Protein Atlas
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
Contribution from Antibody Resource (www.antibodyresource.com) — January 18, 2017
Antibody Resource is spearheading an initiative designed to compare antibodies from numerous suppliers using identical samples/tissues and an identical protocol. In doing so, they hope to enable scientists to form an unrivalled opinion of which is the most suitable antibody for their research and which is going to require the least amount of optimization, a process which can often take weeks or months.
For the purposes of the antibody comparison initiative, the best antibodies from each manufacturer were selected and compared side-by-side using the same experimental conditions to provide a direct comparison. The antibodies were collected centrally, repackaged and given an internal reference ID prior to delivery to independent laboratories to ensure objective testing and to minimize bias.
Anti-NALP3 AMAb90569 antibody (M80 in the test) exhibited immunoreactivity in both cell lysates with bands around the expected MW of 118kDa. Anti-NALP3 AMAb90569 was one of only two antibodies that showed supportive results in the test.
Western blot analysis in 1. THP1 whole cell lysate, 2. Jurkat whole cell lysate using Anti-NLRP3 Antibody (AMAb90569).
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