Enhanced Validation

Monoclonal Anti-NAPRT Antibody

nicotinate phosphoribosyltransferase
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human NAPRT
Alternative Gene Names
NAPRT1, PP3856
Open Datasheet
Price
$505.00
Product Number
AMAb90725
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[5 pictures]
Immunohistochemistry analysis in human duodenum and cerebral cortex tissues using AMAb90725 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Lane 1: Marker [kDa]
Lane 2: Human liver tissue lysate

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
nicotinate phosphoribosyltransferase
Target Gene
NAPRT
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000022574 (86%)

Rat ENSRNOG00000007939 (82%)

Clonality
Monoclonal
Clone ID
CL0366
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90725 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
nicotinate phosphoribosyltransferase
Gene Name
NAPRT
Alternative Gene Names
NAPRT1, PP3856
UniProt ID
Q6XQN6
Gene (Ensembl)
ENSG00000147813
Entrez Gene ID
Entrez gene: 93100
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Managò A, Audrito V, Mazzola F, Sorci L, Gaudino F, Gizzi K, Vitale N, Incarnato D, Minazzato G, Ianniello A, Varriale A, D’Auria S, Mengozzi G, Politano G, Oliviero S, Raffaelli N, Deaglio S

Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation

Nat Commun , 2019 Sep 11; 10:4116. Epub 2019 Sep 11

PubMed ID: 31511522 DOI: 10.1038/s41467-019-12055-2

Cole J, Guiot MC, Gravel M, Bernier C, Shore GC, Roulston A

Novel NAPRT specific antibody identifies small cell lung cancer and neuronal cancers as promising clinical indications for a NAMPT inhibitor/niacin co-administration strategy

Oncotarget , 2017 Sep 12; 8(44):77846-77859. Epub 2017 Sep 12

PubMed ID: 29100430 DOI: 10.18632/oncotarget.20840

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-NAPRT (AMAb90823)
Anti-NAPRT (HPA023739)
Anti-NAPRT (HPA024017)
Corresponding Antigens
PrEST Antigen NAPRT (APrEST76286)

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