Monoclonal Anti-NANOG Antibody

Nanog homeobox
References (1)
Recommended Applications
Product Description
Monoclonal Antibody against Human NANOG
Alternative Gene Names
FLJ12581, FLJ40451
Open Datasheet
Price
$505.00
Product Number
AMAb91393
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[3 pictures]
Immunohistochemical staining of human testis cancer shows strong nuclear immunoreactivity in tumor cells.
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[1 pictures]
Western blot analysis in human cell line NTERA-2.
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[1 pictures]
Immunofluorescence staining of NTERA-2 cells using the Anti-NANOG monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Nanog homeobox
Target Gene
NANOG
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000012396 (100%)

Rat ENSRNOG00000008368 (80%)

Clonality
Monoclonal
Clone ID
CL5810
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91393 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91393 on the Human Protein Atlas
Protein Name
Nanog homeobox
Gene Name
NANOG
Alternative Gene Names
FLJ12581, FLJ40451
UniProt ID
Q9H9S0
Gene (Ensembl)
ENSG00000111704
Entrez Gene ID
Entrez gene: 79923
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Bååth M, Jönsson JM, Westbom Fremer S, Martín de la Fuente L, Tran L, Malander S, Kannisto P, Måsbäck A, Honeth G, Hedenfalk I

MET Expression and Cancer Stem Cell Networks Impact Outcome in High-Grade Serous Ovarian Cancer.

Genes (Basel) , 2021 May 14; 12(5):None

PubMed ID: 34069138 DOI: 10.3390/genes12050742

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-NANOG (AMAb91391)
Anti-NANOG (HPA072117)

Questions?

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