Enhanced Validation

Monoclonal Anti-MLANA Antibody

Melan-a
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.
Product Description
Monoclonal Antibody against Human MLANA
Alternative Gene Names
MART1
Open Datasheet
Price
$379.00
Product Number
AMAb91816
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human skin and prostate tissues using AMAb91816 antibody. Corresponding MLANA RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in human cell line SK-MEL-30 and human cell line U-2 OS.
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[1 pictures]
Immunofluorescence staining of SK-MEL-30 cells using the Anti-MART1 monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Melan-a
Target Gene
MLANA
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000036608 (67%)

Mouse ENSMUSG00000024806 (64%)

Clonality
Monoclonal
Clone ID
CL12863
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
25 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91816 on the Human Protein Atlas
Western Blot (WB)

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91816 on the Human Protein Atlas
Protein Name
Melan-a
Gene Name
MLANA
Alternative Gene Names
MART1
UniProt ID
Q16655
Gene (Ensembl)
ENSG00000120215
Entrez Gene ID
Entrez gene: 2315
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Did we miss your publication?

Have you published using AMAb91816? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-MLANA (AMAb91817)
Anti-MLANA (AMAb91818)
Anti-MLANA (HPA048662)
Corresponding Antigens
PrEST Antigen MLANA (APrEST81897)

Questions?

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