Monoclonal Anti-MKL2 Antibody

MKL/myocardin-like 2
References (1)
Recommended Applications
Product Description
Monoclonal Antibody against Human MKL2
Alternative Gene Names
FLJ31823, MRTF-B
Open Datasheet
Price
$505.00
Product Number
AMAb90887
Unit Size
Concentration
Lot dependent
Availability
4 in Stock
Ships
Ready to Ship
Show all images
[1 pictures]
Western blot analysis in human cell line SiHa.
Show all images
[2 pictures]
Immunofluorescence staining of A549 cells using the Anti-MKL2 monoclonal antibody, showing specific staining in nuclear speckles and the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
MKL/myocardin-like 2
Target Gene
MKL2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000009569 (89%)

Rat ENSRNOG00000018803 (54%)

Clonality
Monoclonal
Clone ID
CL1546
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90887 on the Human Protein Atlas
Protein Name
MKL/myocardin-like 2
Gene Name
MKL2
Alternative Gene Names
FLJ31823, MRTF-B
UniProt ID
Q9ULH7
Gene (Ensembl)
ENSG00000186260
Entrez Gene ID
Entrez gene: 57496
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Hadden WJ, Young JL, Holle AW, McFetridge ML, Kim DY, Wijesinghe P, Taylor-Weiner H, Wen JH, Lee AR, Bieback K, Vo BN, Sampson DD, Kennedy BF, Spatz JP, Engler AJ, Choi YS

Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels

Proc Natl Acad Sci U S A , 2017 May 15; 114(22):5647-5652. Epub 2017 May 15

PubMed ID: 28507138 DOI: 10.1073/pnas.1618239114

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens
PrEST Antigen MKL2 (APrEST71003)

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