Enhanced Validation

Monoclonal Anti-MCU Antibody

mitochondrial calcium uniporter
References (2)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human MCU
Alternative Gene Names
C10orf42, CCDC109A, FLJ46135
Open Datasheet
Price
$505.00
Product Number
AMAb91189
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[1 pictures]
Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MCU antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Show all images
[1 pictures]
Immunofluorescence staining of A549 cells using the Anti-MCU monoclonal antibody, showing specific staining in the mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
mitochondrial calcium uniporter
Target Gene
MCU
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000009647 (100%)

Rat ENSRNOG00000045920 (100%)

Clonality
Monoclonal
Clone ID
CL3576
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91189 on the Human Protein Atlas
Protein Name
mitochondrial calcium uniporter
Gene Name
MCU
Alternative Gene Names
C10orf42, CCDC109A, FLJ46135
UniProt ID
Q8NE86
Gene (Ensembl)
ENSG00000156026
Entrez Gene ID
Entrez gene: 90550
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Canato M, Capitanio P, Cancellara L, Leanza L, Raffaello A, Reane DV, Marcucci L, Michelucci A, Protasi F, Reggiani C

Excessive Accumulation of Ca2 + in Mitochondria of Y522S-RYR1 Knock-in Mice: A Link Between Leak From the Sarcoplasmic Reticulum and Altered Redox State

Front Physiol , 2019 Sep 13; 10:1142. Epub 2019 Sep 13

PubMed ID: 31607937 DOI: 10.3389/fphys.2019.01142

Gu L, Larson Casey JL, Andrabi SA, Lee JH, Meza-Perez S, Randall TD, Carter AB

Mitochondrial calcium uniporter regulates PGC-1α expression to mediate metabolic reprogramming in pulmonary fibrosis

Redox Biol , 2019 Aug 25; 26:101307. Epub 2019 Aug 25

PubMed ID: 31473487 DOI: 10.1016/j.redox.2019.101307

Did we miss your publication?

Have you published using AMAb91189? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Corresponding Antigens
PrEST Antigen MCU (APrEST93522)

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