Monoclonal Anti-MCL1 Antibody

myeloid cell leukemia sequence 1 (BCL2-related)
Recommended Applications
Product Description
Monoclonal Antibody against Human MCL1
Alternative Gene Names
BCL2L3, Mcl-1
Open Datasheet
Price
$505.00
Product Number
AMAb90859
Unit Size
Concentration
Lot dependent
Availability
8 in Stock
Ships
Ready to Ship
Show all images
[5 pictures]
Immunohistochemical staining of human tonsil shows strong immunoreactivity in the reaction centrum.
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[1 pictures]
Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate) 
Lane 3: MCL1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411855)
Show all images
[1 pictures]
Immunofluorescence staining of U-2 OS cells using the Anti-MCL1 monoclonal antibody, showing cell cycle dependent staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
myeloid cell leukemia sequence 1 (BCL2-related)
Target Gene
MCL1
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000038612 (77%)

Rat ENSRNOG00000023527 (58%)

Clonality
Monoclonal
Clone ID
CL1128
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90859 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90859 on the Human Protein Atlas
Protein Name
myeloid cell leukemia sequence 1 (BCL2-related)
Gene Name
MCL1
Alternative Gene Names
BCL2L3, Mcl-1
UniProt ID
Q07820
Gene (Ensembl)
ENSG00000143384
Entrez Gene ID
Entrez gene: 4170
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-MCL1 (HPA008455)
Anti-MCL1 (HPA031125)
Corresponding Antigens
PrEST Antigen MCL1 (APrEST70113)

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