Enhanced Validation

Monoclonal Anti-MBP Antibody

myelin basic protein
References (5)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human MBP
Open Datasheet
Price
$505.00
Product Number
AMAb91064
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[10 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb91064 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.
Show all images
[2 pictures]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
myelin basic protein
Target Gene
MBP
Belongs to Antibody Panel
Neuroscience Markers
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000041607 (97%)

Rat ENSRNOG00000016516 (97%)

Clonality
Monoclonal
Clone ID
CL2829
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91064 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
myelin basic protein
Gene Name
MBP
UniProt ID
P02686
Gene (Ensembl)
ENSG00000197971
Entrez Gene ID
Entrez gene: 4155
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Schäffner E, Bosch-Queralt M, Edgar JM, Lehning M, Strauß J, Fleischer N, Kungl T, Wieghofer P, Berghoff SA, Reinert T, Krueger M, Morawski M, Möbius W, Barrantes-Freer A, Stieler J, Sun T, Saher G, Schwab MH, Wrede C, Frosch M, Prinz M, Reich DS, Flügel A, Stadelmann C, Fledrich R, Nave KA, Stassart RM

Myelin insulation as a risk factor for axonal degeneration in autoimmune demyelinating disease

Nat Neurosci , 2023 Jun 29; 26(7):1218-1228. Epub 2023 Jun 29

PubMed ID: 37386131 DOI: 10.1038/s41593-023-01366-9

Woelfle S, Deshpande D, Feldengut S, Braak H, Del Tredici K, Roselli F, Deisseroth K, Michaelis J, Boeckers TM, Schön M

CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue

BMC Biol , 2023 May 24; 21:113. Epub 2023 May 24

PubMed ID: 37221592 DOI: 10.1186/s12915-023-01582-6

Zhao S, Todorov MI, Cai R, AI-Maskari R, Steinke H, Kemter E, Mai H, Rong Z, Warmer M, Stanic K, Schoppe O, Paetzold JC, Gesierich B, Wong MN, Huber TB, Duering M, Bruns OT, Menze B, Lipfert J, Puelles VG, Wolf E, Bechmann I, Ertürk A

Cellular and Molecular Probing of Intact Human Organs

Cell , 2020 Feb 13; 180(4):796-812.e19. Epub 2020 Feb 13

PubMed ID: 32059778 DOI: 10.1016/j.cell.2020.01.030

Dolci S, Pino A, Berton V, Gonzalez P, Braga A, Fumagalli M, Bonfanti E, Malpeli G, Pari F, Zorzin S, Amoroso C, Moscon D, Rodriguez FJ, Fumagalli G, Bifari F, Decimo I

High Yield of Adult Oligodendrocyte Lineage Cells Obtained from Meningeal Biopsy

Front Pharmacol , 2017 Oct 12; 8:703. Epub 2017 Oct 12

PubMed ID: 29075188 DOI: 10.3389/fphar.2017.00703

Ly H, Verma N, Wu F, Liu M, Saatman KE, Nelson PT, Slevin JT, Goldstein LB, Biessels GJ, Despa F

Brain microvascular injury and white matter disease provoked by diabetes-associated hyperamylinemia

Ann Neurol , 2017 Jul 29; 82(2):208-222. Epub 2017 Jul 29

PubMed ID: 28696548 DOI: 10.1002/ana.24992

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