Anti-LAPTM4B Antibody

lysosomal protein transmembrane 4 beta
Recommended Applications
Product Description
Monoclonal Antibody against Human LAPTM4B
Alternative Gene Names
LC27
Price
$437.00
Product Number
AMAb91356
Unit Size
100 µl
Concentration
Lot dependent
Availability
10 in Stock
Ships
Ready to Ship
Target protein
lysosomal protein transmembrane 4 beta
Target gene
LAPTM4B
Antigen sequence
Synthetic Peptide
EYIRQLPPNFPYRDD
Verified Species Reactivity
Human
Clonality
Monoclonal
Clone ID
CL5289
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
[This part should be hidden or updated after call to Current Batch]
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in cell lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 1-4 µg/ml

Protocols
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
lysosomal protein transmembrane 4 beta
Gene Name
LAPTM4B
Alternative Gene Names
LC27
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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User Data Western Blot, Customer Submission

Contribution from Dr. Tomas Blom, University of Helsinki — May 30, 2017

We have validated the Anti-LAPTM4B antibody (AMAb91356) by Western blot using cell lysates of A431 (human epidermoid carcinoma cell line).

AMAb91356_wb

40µg of protein was run in each lane. Blocking was done with 5% milk in TBS + 0.1% Tween. Primary (AMAb91356) and secondary (Goat anti mouse-HRP) antibodies were diluted 1:1000 in blocking solution.

Lane 1. A431 wild type

Lane 2. A431 LAPTM4B-/-

Lane 3. LAPTM4B long isoform (NP_060877.3) with C-terminal 3xFLAG overexpressed in A431 LAPTM4B-/- cells

Lane 4. LAPTM4B short isoform (AAH14129.1) with C-terminal 3xFLAG expressed in A431 LAPTM4B-/- cells

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not articipate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 50% of the product price as a refund, or 50% discount on your next vial purchase.

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