Enhanced Validation

Monoclonal Anti-LAPTM4B Antibody

Lysosomal protein transmembrane 4 beta
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human LAPTM4B
Alternative Gene Names
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
Lysosomal protein transmembrane 4 beta
Target Gene
Verified Species Reactivity
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000022257 (93%)

Rat ENSRNOG00000006766 (93%)

Clone ID
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
Lysosomal protein transmembrane 4 beta
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Yan R, Liu D, Wang J, Liu M, Guo H, Bai J, Yang S, Chang J, Yao Z, Yang Z, Blom T, Zhou K

miR-137–LAPTM4B regulates cytoskeleton organization and cancer metastasis via the RhoA-LIMK-Cofilin pathway in osteosarcoma

Oncogenesis , 2023 May 6; 12(1):25. Epub 2023 May 6

PubMed ID: 37147294 DOI: 10.1038/s41389-023-00471-5

Liu M, Yan R, Wang J, Yao Z, Fan X, Zhou K

LAPTM4B‐35 promotes cancer cell migration via stimulating integrin beta1 recycling and focal adhesion dynamics

Cancer Sci , 2022 Apr 19; 113(6):2022-2033. Epub 2022 Apr 19

PubMed ID: 35381120 DOI: 10.1111/cas.15362

Zhou K, Dichlberger A, Ikonen E, Blom T

Lysosome Associated Protein Transmembrane 4B-24 Is the Predominant Protein Isoform in Human Tissues and Undergoes Rapid, Nutrient-Regulated Turnover.

Am J Pathol , 2020 Oct; 190(10):2018-2028

PubMed ID: 32679228 DOI: https://doi.org/10.1016/j.ajpath.2020.07.003

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User Data Western Blot, Customer Submission

Contribution from Dr. Tomas Blom, University of Helsinki — May 30, 2017

We have validated the Anti-LAPTM4B antibody (AMAb91356) by Western blot using cell lysates of A431 (human epidermoid carcinoma cell line).


40µg of protein was run in each lane. Blocking was done with 5% milk in TBS + 0.1% Tween. Primary (AMAb91356) and secondary (Goat anti mouse-HRP) antibodies were diluted 1:1000 in blocking solution.

Lane 1. A431 wild type

Lane 2. A431 LAPTM4B-/-

Lane 3. LAPTM4B long isoform (NP_060877.3) with C-terminal 3xFLAG overexpressed in A431 LAPTM4B-/- cells

Lane 4. LAPTM4B short isoform (AAH14129.1) with C-terminal 3xFLAG expressed in A431 LAPTM4B-/- cells

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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