Anti-LAPTM4B Antibody



Human Protein Atlas
Recommended conditions:
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Recommended conditions:
Working concentration: 1 µg/ml
Recommended conditions:
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
Did we miss your publication?
Have you published using AMAb91356? Please let us know and we will be happy to include your reference on this page.
Contribution from Dr. Tomas Blom, University of Helsinki — May 30, 2017
We have validated the Anti-LAPTM4B antibody (AMAb91356) by Western blot using cell lysates of A431 (human epidermoid carcinoma cell line).
40µg of protein was run in each lane. Blocking was done with 5% milk in TBS + 0.1% Tween. Primary (AMAb91356) and secondary (Goat anti mouse-HRP) antibodies were diluted 1:1000 in blocking solution.
Lane 1. A431 wild type
Lane 2. A431 LAPTM4B-/-
Lane 3. LAPTM4B long isoform (NP_060877.3) with C-terminal 3xFLAG overexpressed in A431 LAPTM4B-/- cells
Lane 4. LAPTM4B short isoform (AAH14129.1) with C-terminal 3xFLAG expressed in A431 LAPTM4B-/- cells
Join the Explorer Program
Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers 50% of the product price as a refund, or 50% discount on your next vial purchase.
Questions?
Our customer support and expert scientific support teams are here to help you succeed in your research.