Human Protein Atlas
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 1-4 µg/ml
Validated in Western blot using relevant lysates (see Western blot application images above)
Contribution from Dr. Tomas Blom, University of Helsinki — May 30, 2017
We have validated the Anti-LAPTM4B antibody (AMAb91356) by Western blot using cell lysates of A431 (human epidermoid carcinoma cell line).
40µg of protein was run in each lane. Blocking was done with 5% milk in TBS + 0.1% Tween. Primary (AMAb91356) and secondary (Goat anti mouse-HRP) antibodies were diluted 1:1000 in blocking solution.
Lane 1. A431 wild type
Lane 2. A431 LAPTM4B-/-
Lane 3. LAPTM4B long isoform (NP_060877.3) with C-terminal 3xFLAG overexpressed in A431 LAPTM4B-/- cells
Lane 4. LAPTM4B short isoform (AAH14129.1) with C-terminal 3xFLAG expressed in A431 LAPTM4B-/- cells
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