Monoclonal Anti-LAPTM4B Antibody
Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000022257 (93%)
Rat ENSRNOG00000006766 (93%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
miR-137–LAPTM4B regulates cytoskeleton organization and cancer metastasis via the RhoA-LIMK-Cofilin pathway in osteosarcoma
Oncogenesis , 2023 May 6; 12(1):25. Epub 2023 May 6
LAPTM4B‐35 promotes cancer cell migration via stimulating integrin beta1 recycling and focal adhesion dynamics
Cancer Sci , 2022 Apr 19; 113(6):2022-2033. Epub 2022 Apr 19
Lysosome Associated Protein Transmembrane 4B-24 Is the Predominant Protein Isoform in Human Tissues and Undergoes Rapid, Nutrient-Regulated Turnover.
Am J Pathol , 2020 Oct; 190(10):2018-2028
PubMed ID: 32679228 DOI: https://doi.org/10.1016/j.ajpath.2020.07.003
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Contribution from Dr. Tomas Blom, University of Helsinki — May 30, 2017
We have validated the Anti-LAPTM4B antibody (AMAb91356) by Western blot using cell lysates of A431 (human epidermoid carcinoma cell line).
40µg of protein was run in each lane. Blocking was done with 5% milk in TBS + 0.1% Tween. Primary (AMAb91356) and secondary (Goat anti mouse-HRP) antibodies were diluted 1:1000 in blocking solution.
Lane 1. A431 wild type
Lane 2. A431 LAPTM4B-/-
Lane 3. LAPTM4B long isoform (NP_060877.3) with C-terminal 3xFLAG overexpressed in A431 LAPTM4B-/- cells
Lane 4. LAPTM4B short isoform (AAH14129.1) with C-terminal 3xFLAG expressed in A431 LAPTM4B-/- cells
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