Enhanced Validation

Monoclonal Anti-LAPTM4B Antibody

Lysosomal protein transmembrane 4 beta
References (3) Researcher contributions (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human LAPTM4B
Alternative Gene Names
LC27
Open Datasheet
Price
$505.00
Product Number
AMAb91356
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human heart muscle and tonsil tissues using AMAb91356 antibody. Corresponding LAPTM4B RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in human cell line HEL.
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[1 pictures]
Immunofluorescence staining of A-431 cells using the Anti-LAPTM4B monoclonal antibody, showing specific staining in vesicles and the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Lysosomal protein transmembrane 4 beta
Target Gene
LAPTM4B
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000022257 (93%)

Rat ENSRNOG00000006766 (93%)

Clonality
Monoclonal
Clone ID
CL5289
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91356 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91356 on the Human Protein Atlas
Protein Name
Lysosomal protein transmembrane 4 beta
Gene Name
LAPTM4B
Alternative Gene Names
LC27
UniProt ID
Q86VI4
Gene (Ensembl)
ENSG00000104341
Entrez Gene ID
Entrez gene: 55353
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Yan R, Liu D, Wang J, Liu M, Guo H, Bai J, Yang S, Chang J, Yao Z, Yang Z, Blom T, Zhou K

miR-137–LAPTM4B regulates cytoskeleton organization and cancer metastasis via the RhoA-LIMK-Cofilin pathway in osteosarcoma

Oncogenesis , 2023 May 6; 12(1):25. Epub 2023 May 6

PubMed ID: 37147294 DOI: 10.1038/s41389-023-00471-5

Liu M, Yan R, Wang J, Yao Z, Fan X, Zhou K

LAPTM4B‐35 promotes cancer cell migration via stimulating integrin beta1 recycling and focal adhesion dynamics

Cancer Sci , 2022 Apr 19; 113(6):2022-2033. Epub 2022 Apr 19

PubMed ID: 35381120 DOI: 10.1111/cas.15362

Zhou K, Dichlberger A, Ikonen E, Blom T

Lysosome Associated Protein Transmembrane 4B-24 Is the Predominant Protein Isoform in Human Tissues and Undergoes Rapid, Nutrient-Regulated Turnover.

Am J Pathol , 2020 Oct; 190(10):2018-2028

PubMed ID: 32679228 DOI: https://doi.org/10.1016/j.ajpath.2020.07.003

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User Data Western Blot, Customer Submission

Contribution from Dr. Tomas Blom, University of Helsinki — May 30, 2017

We have validated the Anti-LAPTM4B antibody (AMAb91356) by Western blot using cell lysates of A431 (human epidermoid carcinoma cell line).

AMAb91356_wb

40µg of protein was run in each lane. Blocking was done with 5% milk in TBS + 0.1% Tween. Primary (AMAb91356) and secondary (Goat anti mouse-HRP) antibodies were diluted 1:1000 in blocking solution.

Lane 1. A431 wild type

Lane 2. A431 LAPTM4B-/-

Lane 3. LAPTM4B long isoform (NP_060877.3) with C-terminal 3xFLAG overexpressed in A431 LAPTM4B-/- cells

Lane 4. LAPTM4B short isoform (AAH14129.1) with C-terminal 3xFLAG expressed in A431 LAPTM4B-/- cells

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-LAPTM4B (AMAb91355)
Anti-LAPTM4B (HPA068830)

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