Monoclonal Anti-LAPTM4B Antibody
Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Mouse ENSMUSG00000022257 (93%)
Rat ENSRNOG00000006766 (93%)
Dilution: 1:500 - 1:1000
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
LAPTM4B‐35 promotes cancer cell migration via stimulating integrin beta1 recycling and focal adhesion dynamics
Cancer Sci , 2022 Apr 19; 113(6):2022-2033. Epub 2022 Apr 19
Lysosome Associated Protein Transmembrane 4B-24 Is the Predominant Protein Isoform in Human Tissues and Undergoes Rapid, Nutrient-Regulated Turnover.
Am J Pathol , 2020 Oct; 190(10):2018-2028
Contribution from Dr. Tomas Blom, University of Helsinki — May 30, 2017
We have validated the Anti-LAPTM4B antibody (AMAb91356) by Western blot using cell lysates of A431 (human epidermoid carcinoma cell line).
40µg of protein was run in each lane. Blocking was done with 5% milk in TBS + 0.1% Tween. Primary (AMAb91356) and secondary (Goat anti mouse-HRP) antibodies were diluted 1:1000 in blocking solution.
Lane 1. A431 wild type
Lane 2. A431 LAPTM4B-/-
Lane 3. LAPTM4B long isoform (NP_060877.3) with C-terminal 3xFLAG overexpressed in A431 LAPTM4B-/- cells
Lane 4. LAPTM4B short isoform (AAH14129.1) with C-terminal 3xFLAG expressed in A431 LAPTM4B-/- cells
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