Monoclonal Anti-KDM5B Antibody
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401985)
Human Protein Atlas
Highest antigen sequence identity to the following orthologs:
Rat ENSRNOG00000004433 (91%)
Mouse ENSMUSG00000042207 (86%)
Dilution: 1:200 - 1:500
Retrieval method: HIER pH6
Working concentration: 1 µg/ml
Fixation/Permeabilization: PFA/Triton X-100
Working concentration: 2-10 µg/ml
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Validated in Western blot using relevant lysates (see Western blot application images above)
Profiling cancer testis antigens in non–small-cell lung cancer
JCI Insight , 2016 Jul 7; 1(10):e86837. Epub 2016 Jul 7
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