Enhanced Validation

Monoclonal Anti-ITGA3 Antibody

integrin subunit alpha 3
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human ITGA3
Alternative Gene Names
CD49c, GAP-B3, MSK18, VCA-2, VLA3a
Open Datasheet
Price
$505.00
Product Number
AMAb91446
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human lung and liver tissues using AMAb91446 antibody. Corresponding ITGA3 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ITGA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
integrin subunit alpha 3
Target Gene
ITGA3
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000001507 (84%)

Rat ENSRNOG00000004276 (82%)

Clonality
Monoclonal
Clone ID
CL6937
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:100 - 1:250

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91446 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
integrin subunit alpha 3
Gene Name
ITGA3
Alternative Gene Names
CD49c, GAP-B3, MSK18, VCA-2, VLA3a
UniProt ID
P26006
Gene (Ensembl)
ENSG00000005884
Entrez Gene ID
Entrez gene: 3675
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-ITGA3 (HPA008572)
Corresponding Antigens
PrEST Antigen ITGA3 (APrEST86765)

Questions?

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