Enhanced Validation

Monoclonal Anti-HMGCR Antibody

3-hydroxy-3-methylglutaryl-coa reductase
References (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human HMGCR
Open Datasheet
Price
$505.00
Product Number
AMAb90619
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human liver and skeletal muscle tissues using AMAb90619 antibody. Corresponding HMGCR RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HMGCR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
3-hydroxy-3-methylglutaryl-coa reductase
Target Gene
HMGCR
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000021670 (94%)

Rat ENSRNOG00000016122 (92%)

Clonality
Monoclonal
Clone ID
CL0260
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20 - 1:50

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90619 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
3-hydroxy-3-methylglutaryl-coa reductase
Gene Name
HMGCR
UniProt ID
P04035
Gene (Ensembl)
ENSG00000113161
Entrez Gene ID
Entrez gene: 3156
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Kimbung S, Lettiero B, Feldt M, Bosch A, Borgquist S

High expression of cholesterol biosynthesis genes is associated with resistance to statin treatment and inferior survival in breast cancer

Oncotarget , 2016 Jul 21; 7(37):59640-59651. Epub 2016 Jul 21

PubMed ID: 27458152 DOI: 10.18632/oncotarget.10746

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Have you published using AMAb90619? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-HMGCR (AMAb90618)
Anti-HMGCR (HPA008338)

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