Monoclonal Anti-HIST1H3A Antibody

Histone cluster 1 h3 family member a
Recommended Applications
Product Description
Monoclonal Antibody against Human HIST1H3A
Alternative Gene Names
H3, H3FA, H3FB, H3FC, H3FD, H3FF, H3FHH3FI, H3FJ, H3FK, H3FL
Open Datasheet
Price
$505.00
Product Number
AMAb91331
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.
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[3 pictures]
Western blot analysis in human cell line HeLa.
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[1 pictures]
Immunofluorescence staining of U-2 OS cells using the Anti-HIST1H3A monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Histone cluster 1 h3 family member a
Target Gene
HIST1H3A
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000069265 (100%)

Rat ENSRNOG00000046434 (100%)

Clonality
Monoclonal
Clone ID
CL4967
Isotype
IgG2a
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:10000 - 1:20000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91331 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91331 on the Human Protein Atlas
Protein Name
Histone cluster 1 h3 family member a
Gene Name
HIST1H3A
Alternative Gene Names
H3, H3FA, H3FB, H3FC, H3FD, H3FF, H3FHH3FI, H3FJ, H3FK, H3FL
UniProt ID
P68431
Gene (Ensembl)
ENSG00000275714
Entrez Gene ID
Entrez gene: 8350
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Alternative Antibodies
Anti-HIST1H3A (AMAb91332)

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