Monoclonal Anti-H2AFX Antibody

H2a histone family member x
Recommended Applications
Product Description
Monoclonal Antibody against Human H2AFX
Alternative Gene Names
H2AX, H2A.X
Open Datasheet
Price
$505.00
Product Number
AMAb91346
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[4 pictures]
Immunohistochemical staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.
Show all images
[1 pictures]
Immunofluorescence staining of MCF7 cells using the Anti-H2AFX monoclonal antibody, showing specific staining in nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
H2a histone family member x
Target Gene
H2AFX
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000010386 (88%)

Mouse ENSMUSG00000049932 (81%)

Clonality
Monoclonal
Clone ID
CL5157
Isotype
IgG2a
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20000 - 1:50000

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Protein Name
H2a histone family member x
Gene Name
H2AFX
Alternative Gene Names
H2AX, H2A.X
UniProt ID
P16104
Gene (Ensembl)
ENSG00000188486
Entrez Gene ID
Entrez gene: 3014
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-H2AFX (HPA041189)
Anti-H2AFX (HPA051647)

Questions?

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