Enhanced Validation

Monoclonal Anti-GRHL2 Antibody

grainyhead-like 2 (Drosophila)
References (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human GRHL2
Alternative Gene Names
BOM, DFNA28, FLJ13782, TFCP2L3
Open Datasheet
Price
$505.00
Product Number
AMAb91226
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human prostate and liver tissues using AMAb91226 antibody. Corresponding GRHL2 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Immunofluorescence staining of A-431 cells using the Anti-GRHL2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
grainyhead-like 2 (Drosophila)
Target Gene
GRHL2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000022286 (87%)

Rat ENSRNOG00000007000 (87%)

Clonality
Monoclonal
Clone ID
CL3760
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91226 on the Human Protein Atlas
Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91226 on the Human Protein Atlas
Protein Name
grainyhead-like 2 (Drosophila)
Gene Name
GRHL2
Alternative Gene Names
BOM, DFNA28, FLJ13782, TFCP2L3
UniProt ID
Q6ISB3
Gene (Ensembl)
ENSG00000083307
Entrez Gene ID
Entrez gene: 79977
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
MacFawn I, Wilson H, Selth LA, Leighton I, Serebriiskii I, Bleackley RC, Elzamzamy O, Farris J, Pifer PM, Richer J, Frisch SM

GRAINYHEAD-LIKE-2 CONFERS NK-SENSITIVITY THROUGH INTERACTIONS WITH EPIGENETIC MODIFIERS

Mol Immunol , 2018 Nov 30; 105:137-149. Epub 2018 Nov 30

PubMed ID: 30508726 DOI: 10.1016/j.molimm.2018.11.006

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-GRHL2 (HPA004820)
Anti-GRHL2 (HPA062839)
Corresponding Antigens
PrEST Antigen GRHL2 (APrEST86696)

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