Enhanced Validation

Monoclonal Anti-GORASP2 Antibody

Golgi reassembly stacking protein 2
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human GORASP2
Alternative Gene Names
GOLPH6, GRASP55, GRS2
Open Datasheet
Price
$505.00
Product Number
AMAb91016
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human epididymis and skeletal muscle tissues using AMAb91016 antibody. Corresponding GORASP2 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-GORASP2 monoclonal antibody, showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Golgi reassembly stacking protein 2
Target Gene
GORASP2
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000014959 (68%)

Rat ENSRNOG00000023386 (68%)

Clonality
Monoclonal
Clone ID
CL2610
Isotype
IgG2b
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91016 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91016 on the Human Protein Atlas
Protein Name
Golgi reassembly stacking protein 2
Gene Name
GORASP2
Alternative Gene Names
GOLPH6, GRASP55, GRS2
UniProt ID
Q9H8Y8
Gene (Ensembl)
ENSG00000115806
Entrez Gene ID
Entrez gene: 26003
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Banfi C, Baetta R, Barbieri SS, Brioschi M, Guarino A, Ghilardi S, Sandrini L, Eligini S, Polvani G, Bergman O, Eriksson P, Tremoli E

Prenylcysteine oxidase 1, an emerging player in atherosclerosis

Commun Biol , 2021 Sep 21; 4:1109. Epub 2021 Sep 21

PubMed ID: 34548610 DOI: 10.1038/s42003-021-02630-z

Yeung AT, Choi YH, Lee AH, Hale C, Ponstingl H, Pickard D, Goulding D, Thomas M, Gill E, Kim JK, Bradley A, Hancock RE, Dougan G

A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection

mBio , 2019 Oct 8; 10(5):e02169-19. Epub 2019 Oct 8

PubMed ID: 31594818 DOI: 10.1128/mBio.02169-19

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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