Enhanced Validation

Monoclonal Anti-GFAP Antibody

glial fibrillary acidic protein
References (10)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human GFAP
Alternative Gene Names
FLJ45472
Open Datasheet
Price
$505.00
Product Number
AMAb91033
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[7 pictures]
Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using AMAb91033 antibody. Corresponding GFAP RNA-seq data are presented for the same tissues.
Show all images
[2 pictures]
Western blot analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GFAP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
glial fibrillary acidic protein
Target Gene
GFAP
Belongs to Antibody Panel
Neuroscience Markers
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000002919 (100%)

Mouse ENSMUSG00000020932 (98%)

Clonality
Monoclonal
Clone ID
CL2713
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91033 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
glial fibrillary acidic protein
Gene Name
GFAP
Alternative Gene Names
FLJ45472
UniProt ID
P14136
Gene (Ensembl)
ENSG00000131095
Entrez Gene ID
Entrez gene: 2670
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Starinets A, Tyrtyshnaia A, Manzhulo I

Anti-Inflammatory Activity of Synaptamide in the Peripheral Nervous System in a Model of Sciatic Nerve Injury

Int J Mol Sci , 2023 Mar 27; 24(7):6273. Epub 2023 Mar 27

PubMed ID: 37047247 DOI: 10.3390/ijms24076273

Yang JL, Fan H, Fu FF, Guo BL, Huang Y, Sun L, Wang WT, Xing JL, Hu XT, Ding YQ, Zhang K, Hu YZ, Wang YZ

Transient neurogenesis in ischemic cortex from Sox2+ astrocytes

Neural Regen Res , 2022 Oct 13; 18(7):1521-1526. Epub 2022 Oct 13

PubMed ID: 36571357 DOI: 10.4103/1673-5374.357910

Ponomarenko A, Tyrtyshnaia A, Ivashkevich D, Ermolenko E, Dyuizen I, Manzhulo I

Synaptamide Modulates Astroglial Activity in Mild Traumatic Brain Injury

Mar Drugs , 2022 Aug 21; 20(8):538. Epub 2022 Aug 21

PubMed ID: 36005540 DOI: 10.3390/md20080538

Soued M, Hamdi L, Ben Rehouma M, Mazoit JX, Benhamou D

Antinociceptive properties of losmapimod in two acute pain models in rats: behavioural analysis, immunohistochemistry, dose response, and comparison with usual analgesic drugs

BJA Open , 2022 Aug 17; 3:100029. Epub 2022 Aug 17

PubMed ID: 37588580 DOI: 10.1016/j.bjao.2022.100029

Manzhulo I, Manzhulo O, Tyrtyshnaia A, Ponomarenko A, Konovalova S, Ermolenko E, Milkina E, Starinets A

Modulation of Hippocampal Astroglial Activity by Synaptamide in Rats with Neuropathic Pain

Brain Sci , 2021 Nov 26; 11(12):1561. Epub 2021 Nov 26

PubMed ID: 34942863 DOI: 10.3390/brainsci11121561

Xu X, Du L, Jiang J, Yang M, Wang Z, Wang Y, Tang T, Fu X, Hao J

Microglial TREM2 Mitigates Inflammatory Responses and Neuronal Apoptosis in Angiotensin II-Induced Hypertension in Middle-Aged Mice

Front Aging Neurosci , 2021 Aug 20; 13:716917. Epub 2021 Aug 20

PubMed ID: 34489683 DOI: 10.3389/fnagi.2021.716917

Huang L, Zhou H, Chen K, Chen X, Yang G

Learning-Dependent Dendritic Spine Plasticity Is Reduced in the Aged Mouse Cortex

Front Neural Circuits , 2020 Nov 26; 14:581435. Epub 2020 Nov 26

PubMed ID: 33324172 DOI: 10.3389/fncir.2020.581435

Paolone G, Falcicchia C, Lovisari F, Kokaia M, Bell WJ, Fradet T, Barbieri M, Wahlberg LU, Emerich DF, Simonato M

Long-Term, Targeted Delivery of GDNF from Encapsulated Cells Is Neuroprotective and Reduces Seizures in the Pilocarpine Model of Epilepsy

J Neurosci , 2019 Mar 13; 39(11):2144-2156

PubMed ID: 30665947 DOI: 10.1523/JNEUROSCI.0435-18.2018

Rybachuk O, Kopach O, Pivneva T, Kyryk V

Isolation of Neural Stem Cells from the embryonic mouse hippocampus for in vitro growth or engraftment into a host tissue

Bio Protoc , 2019 Feb 20; 9(4):e3165. Epub 2019 Feb 20

PubMed ID: 33654971 DOI: 10.21769/BioProtoc.3165

Liu Y, Sorce S, Nuvolone M, Domange J, Aguzzi A

Lymphocyte activation gene 3 (Lag3) expression is increased in prion infections but does not modify disease progression

Sci Rep , 2018 Oct 2; 8:14600. Epub 2018 Oct 2

PubMed ID: 30279468 DOI: 10.1038/s41598-018-32712-8

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Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-GFAP (HPA056030)
Anti-GFAP (HPA063513)
Corresponding Antigens
PrEST Antigen GFAP (APrEST85954)

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