Enhanced Validation

Anti-GFAP Antibody

glial fibrillary acidic protein
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human GFAP
Alternative Gene Names
Product Number
Unit Size
Lot dependent
>10 in Stock
Ready to Ship
Target Protein
glial fibrillary acidic protein
Target Gene
Belongs to Antibody Panel
Antigen Sequence
Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Binds to an epitope located within the peptide sequence PVQTFSNLQIRETSL as determined by overlapping synthetic peptides.
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000002919 (100%)

Mouse ENSMUSG00000020932 (98%)

Clone ID
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
glial fibrillary acidic protein
Gene Name
Alternative Gene Names
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Normally shipped at ambient temperature
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Huang L, Zhou H, Chen K, Chen X, Yang G

Learning-Dependent Dendritic Spine Plasticity Is Reduced in the Aged Mouse Cortex

Front Neural Circuits , 2020 Nov 26; 14:581435. Epub 2020 Nov 26

PubMed ID: 33324172 DOI: 10.3389/fncir.2020.581435

Paolone G, Falcicchia C, Lovisari F, Kokaia M, Bell WJ, Fradet T, Barbieri M, Wahlberg LU, Emerich DF, Simonato M

Long-Term, Targeted Delivery of GDNF from Encapsulated Cells Is Neuroprotective and Reduces Seizures in the Pilocarpine Model of Epilepsy

J Neurosci , 2019 Mar 13; 39(11):2144-2156

PubMed ID: 30665947 DOI: 10.1523/JNEUROSCI.0435-18.2018

Rybachuk O, Kopach O, Pivneva T, Kyryk V

Isolation of Neural Stem Cells from the embryonic mouse hippocampus for in vitro growth or engraftment into a host tissue

Bio Protoc , 2019 Feb 20; 9(4):e3165. Epub 2019 Feb 20

PubMed ID: 33654971 DOI: 10.21769/BioProtoc.3165

Liu Y, Sorce S, Nuvolone M, Domange J, Aguzzi A

Lymphocyte activation gene 3 (Lag3) expression is increased in prion infections but does not modify disease progression

Sci Rep , 2018 Oct 2; 8:14600. Epub 2018 Oct 2

PubMed ID: 30279468 DOI: 10.1038/s41598-018-32712-8

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Corresponding Antigens


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