Enhanced Validation

Monoclonal Anti-GDF15 Antibody

growth differentiation factor 15
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human GDF15
Alternative Gene Names
MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Open Datasheet
Price
$505.00
Product Number
AMAb90687
Unit Size
Concentration
Lot dependent
Availability
4 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human placenta and skeletal muscle tissues using AMAb90687 antibody. Corresponding GDF15 RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GDF15 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[1 pictures]
Immunofluorescence staining of A549 cells using the Anti-GDF15 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
growth differentiation factor 15
Target Gene
GDF15
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000038508 (53%)

Rat ENSRNOG00000019661 (51%)

Clonality
Monoclonal
Clone ID
CL0328
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:200 - 1:500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90687 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90687 on the Human Protein Atlas
Protein Name
growth differentiation factor 15
Gene Name
GDF15
Alternative Gene Names
MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
UniProt ID
Q99988
Gene (Ensembl)
ENSG00000130513
Entrez Gene ID
Entrez gene: 9518
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Zhang J, Terán G, Popa M, Madapura H, Ladds MJ, Lianoudaki D, Grünler J, Arsenian-Henriksson M, McCormack E, Rottenberg ME, Catrina SB, Laín S, Darekar S

DHODH inhibition modulates glucose metabolism and circulating GDF15, and improves metabolic balance

iScience , 2021 May 1; 24(5):102494. Epub 2021 May 1

PubMed ID: 34113829 DOI: 10.1016/j.isci.2021.102494

Sadasivan SM, Chen Y, Gupta NS, Han X, Bobbitt KR, Chitale DA, Williamson SR, Rundle AG, Tang D, Rybicki BA

The interplay of growth differentiation factor 15 (GDF15) expression and M2 macrophages during prostate carcinogenesis

Carcinogenesis , 2020 Jul 2; 41(8):1074-1082. Epub 2020 Jul 2

PubMed ID: 32614434 DOI: 10.1093/carcin/bgaa065

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-GDF15 (HPA011191)
Anti-GDF15 (HPA070957)
Corresponding Antigens
PrEST Antigen GDF15 (APrEST72494)

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