Enhanced Validation

Monoclonal Anti-GAPDH Antibody

glyceraldehyde-3-phosphate dehydrogenase
References (5)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human GAPDH
Alternative Gene Names
GAPD
Open Datasheet
Price
$505.00
Product Number
AMAb91153
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[4 pictures]
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Show all images
[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Show all images
[1 pictures]
Immunofluorescence staining of A549 cells using the Anti-GAPDH monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
glyceraldehyde-3-phosphate dehydrogenase
Target Gene
GAPDH
Belongs to Antibody Panel
Loading Controls
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000018630 (94%)

Mouse ENSMUSG00000057666 (92%)

Clonality
Monoclonal
Clone ID
CL3266
Isotype
IgG2a
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50000 - 1:100000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91153 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91153 on the Human Protein Atlas
Protein Name
glyceraldehyde-3-phosphate dehydrogenase
Gene Name
GAPDH
Alternative Gene Names
GAPD
UniProt ID
P04406
Gene (Ensembl)
ENSG00000111640
Entrez Gene ID
Entrez gene: 2597
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Rizzo G, Pineda Chavez SE, Vandenkoornhuyse E, Cárdenas Rincón CL, Cento V, Garlatti V, Wozny M, Sammarco G, Di Claudio A, Meanti L, Elangovan S, Romano A, Roda G, Loy L, Dal Buono A, Gabbiadini R, Lovisa S, Rusconi R, Repici A, Armuzzi A, Vetrano S

Pomegranate Extract Affects Gut Biofilm Forming Bacteria and Promotes Intestinal Mucosal Healing Regulating the Crosstalk between Epithelial Cells and Intestinal Fibroblasts

Nutrients , 2023 Apr 5; 15(7):1771. Epub 2023 Apr 5

PubMed ID: 37049615 DOI: 10.3390/nu15071771

Chong X, Zhu L, Yu D, Chen S, Wang G, Yu Q, Ma X, Xu J, Chen H, An H

ZDHHC9 promotes colon tumor growth by inhibiting effector T cells

Oncol Lett , 2022 Nov 10; 25(1):5. Epub 2022 Nov 10

PubMed ID: 36419754 DOI: 10.3892/ol.2022.13591

Long L, Zeng C, Chen H, Zhou T, Wu L, Cai X

ADNCR modulates neural stem cell differentiation and proliferation through the regulation of TCF3 expression

Ann Transl Med , 2020 Aug; 8(15):927

PubMed ID: 32953727 DOI: 10.21037/atm-20-1068

Xiong X, Feng Y, Li L, Yao J, Zhou M, Zhao P, Huang F, Zeng L, Yuan L

Long non-coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4

Oncol Rep , 2020 Mar 4; 43(5):1503-1515. Epub 2020 Mar 4

PubMed ID: 32323846 DOI: 10.3892/or.2020.7530

Li O, Li Z, Tang Q, Li Y, Yuan S, Shen Y, Zhang Z, Li N, Chu K, Lei G

Long Stress Induced Non-Coding Transcripts 5 (LSINCT5) Promotes Hepatocellular Carcinoma Progression Through Interaction with High-Mobility Group AT-hook 2 and MiR-4516

Med Sci Monit , 2018 Nov 25; 24:8510-8523. Epub 2018 Nov 25

PubMed ID: 30472720 DOI: 10.12659/MSM.911179

Did we miss your publication?

Have you published using AMAb91153? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-GAPDH (AMAb91152)
Anti-GAPDH (HPA040067)
Anti-GAPDH (HPA061280)
Corresponding Antigens
PrEST Antigen GAPDH (APrEST81012)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch