Enhanced Validation

Monoclonal Anti-GAPDH Antibody

glyceraldehyde-3-phosphate dehydrogenase
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human GAPDH
Alternative Gene Names
GAPD
Open Datasheet
Price
$505.00
Product Number
AMAb91152
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemical staining of human cerebral cortex shows immunoreactivity in neuropil as well as strong cytoplasmic positivity in a subset of cells.
Show all images
[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GAPDH antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
glyceraldehyde-3-phosphate dehydrogenase
Target Gene
GAPDH
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000018630 (94%)

Mouse ENSMUSG00000057666 (92%)

Clonality
Monoclonal
Clone ID
CL3265
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91152 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
glyceraldehyde-3-phosphate dehydrogenase
Gene Name
GAPDH
Alternative Gene Names
GAPD
UniProt ID
P04406
Gene (Ensembl)
ENSG00000111640
Entrez Gene ID
Entrez gene: 2597
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-GAPDH (AMAb91153)
Anti-GAPDH (HPA040067)
Anti-GAPDH (HPA061280)
Corresponding Antigens
PrEST Antigen GAPDH (APrEST81012)

Questions?

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