Enhanced Validation

Monoclonal Anti-GAD2 Antibody

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human GAD2
Alternative Gene Names
GAD65
Open Datasheet
Price
$505.00
Product Number
AMAb91048
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Show all images
[9 pictures]
Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using AMAb91048 antibody. Corresponding GAD2 RNA-seq data are presented for the same tissues.
Show all images
[2 pictures]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Target Gene
GAD2
Belongs to Antibody Panel
Neuroscience Markers
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000018200 (88%)

Mouse ENSMUSG00000026787 (84%)

Clonality
Monoclonal
Clone ID
CL2736
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:50 - 1:200

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91048 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Gene Name
GAD2
Alternative Gene Names
GAD65
UniProt ID
Q05329
Gene (Ensembl)
ENSG00000136750
Entrez Gene ID
Entrez gene: 2572
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Tesovnik T, Kovač J, Pohar K, Hudoklin S, Dovč K, Bratina N, Trebušak Podkrajšek K, Debeljak M, Veranič P, Bosi E, Piemonti L, Ihan A, Battelino T

Extracellular Vesicles Derived Human-miRNAs Modulate the Immune System in Type 1 Diabetes

Front Cell Dev Biol , 2020 Mar 31; 8:202. Epub 2020 Mar 31

PubMed ID: 32296701 DOI: 10.3389/fcell.2020.00202

Ding Y, Zheng Y, Liu T, Chen T, Wang C, Sun Q, Hua M, Hua T

Changes in GABAergic markers accompany degradation of neuronal function in the primary visual cortex of senescent rats

Sci Rep , 2017 Nov 2; 7:14897. Epub 2017 Nov 2

PubMed ID: 29097694 DOI: 10.1038/s41598-017-15006-3

Did we miss your publication?

Have you published using AMAb91048? Please let us know and we will be happy to include your reference on this page.

Read more

Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

Read more

Alternative Antibodies
Anti-GAD2 (HPA044637)
Corresponding Antigens
PrEST Antigen GAD2 (APrEST80257)

Questions?

Our customer support and expert scientific support teams are here to help you succeed in your research.

Get in touch