Enhanced Validation

Monoclonal Anti-FUS Antibody

fused in sarcoma
References (4)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human FUS
Alternative Gene Names
ALS6, FUS1, hnRNP-P2, TLS
Open Datasheet
Price
$505.00
Product Number
AMAb90549
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb90549 antibody. Corresponding FUS RNA-seq data are presented for the same tissues.
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[3 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-FUS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
fused in sarcoma
Target Gene
FUS
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000030795 (91%)

Rat ENSRNOG00000023360 (91%)

Clonality
Monoclonal
Clone ID
CL0190
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:2500 - 1:5000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90549 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90549 on the Human Protein Atlas
Protein Name
fused in sarcoma
Gene Name
FUS
Alternative Gene Names
ALS6, FUS1, hnRNP-P2, TLS
UniProt ID
P35637
Gene (Ensembl)
ENSG00000089280
Entrez Gene ID
Entrez gene: 2521
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Rosario R, Stewart HL, Choudhury NR, Michlewski G, Charlet‐Berguerand N, Anderson RA

Evidence for a fragile X messenger ribonucleoprotein 1 (FMR1) mRNA gain‐of‐function toxicity mechanism contributing to the pathogenesis of fragile X‐associated premature ovarian insufficiency

FASEB J , 2022 Oct 17; 36(11):e22612. Epub 2022 Oct 17

PubMed ID: 36250920 DOI: 10.1096/fj.202200468RR

Raykova D, Kermpatsou D, Malmqvist T, Harrison PJ, Sander MR, Stiller C, Heldin J, Leino M, Ricardo S, Klemm A, David L, Spjuth O, Vemuri K, Dimberg A, Sundqvist A, Norlin M, Klaesson A, Kampf C, Söderberg O

A method for Boolean analysis of protein interactions at a molecular level

Nat Commun , 2022 Aug 13; 13:4755. Epub 2022 Aug 13

PubMed ID: 35963857 DOI: 10.1038/s41467-022-32395-w

Decker CJ, Burke JM, Mulvaney PK, Parker R

RNA is required for the integrity of multiple nuclear and cytoplasmic membrane‐less RNP granules

EMBO J , 2022 Mar 31; 41(9):e110137. Epub 2022 Mar 31

PubMed ID: 35355287 DOI: 10.15252/embj.2021110137

Marrone L, Drexler HC, Wang J, Tripathi P, Distler T, Heisterkamp P, Anderson EN, Kour S, Moraiti A, Maharana S, Bhatnagar R, Belgard TG, Tripathy V, Kalmbach N, Hosseinzadeh Z, Crippa V, Abo-Rady M, Wegner F, Poletti A, Troost D, Aronica E, Busskamp V, Weis J, Pandey UB, Hyman AA, Alberti S, Goswami A, Sterneckert J

FUS pathology in ALS is linked to alterations in multiple ALS-associated proteins and rescued by drugs stimulating autophagy

Acta Neuropathol , 2019 Apr 1; 138(1):67-84. Epub 2019 Apr 1

PubMed ID: 30937520 DOI: 10.1007/s00401-019-01998-x

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Alternative Antibodies
Anti-FUS (HPA008784)
Corresponding Antigens
PrEST Antigen FUS (APrEST86697)

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