Enhanced Validation

Monoclonal Anti-FBLN1 Antibody

fibulin 1
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human FBLN1
Alternative Gene Names
FBLN
Open Datasheet
Price
$505.00
Product Number
AMAb90696
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemistry analysis in human cervix, uterine and liver tissues using AMAb90696 antibody. Corresponding FBLN1 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-FBLN1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[2 pictures]
Immunofluorescence staining of RT-4 cells using the Anti-FBLN1 monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
fibulin 1
Target Gene
FBLN1
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000014137 (93%)

Mouse ENSMUSG00000006369 (92%)

Clonality
Monoclonal
Clone ID
CL0337
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90696 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90696 on the Human Protein Atlas
Protein Name
fibulin 1
Gene Name
FBLN1
Alternative Gene Names
FBLN
UniProt ID
P23142
Gene (Ensembl)
ENSG00000077942
Entrez Gene ID
Entrez gene: 2192
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Have you published using AMAb90696? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-FBLN1 (HPA001612)
Anti-FBLN1 (HPA001613)
Corresponding Antigens
PrEST Antigen FBLN1 (APrEST84524)

Questions?

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