Enhanced Validation

Monoclonal Anti-EZH2 Antibody

Enhancer of zeste 2 polycomb repressive complex 2 subunit
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human EZH2
Alternative Gene Names
ENX-1, EZH1, KMT6, KMT6A
Open Datasheet
Price
$505.00
Product Number
AMAb91750
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[7 pictures]
Immunohistochemistry analysis in human testis and cerebral cortex tissues using AMAb91750 antibody. Corresponding EZH2 RNA-seq data are presented for the same tissues.
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[1 pictures]
Immunofluorescence staining of A-431 cells using the Anti-EZH2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probe is visualized in red.

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Enhancer of zeste 2 polycomb repressive complex 2 subunit
Target Gene
EZH2
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000029687 (100%)

Rat ENSRNOG00000006048 (100%)

Clonality
Monoclonal
Clone ID
CL11945
Isotype
IgG2a
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91750 on the Human Protein Atlas
Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91750 on the Human Protein Atlas
Protein Name
Enhancer of zeste 2 polycomb repressive complex 2 subunit
Gene Name
EZH2
Alternative Gene Names
ENX-1, EZH1, KMT6, KMT6A
UniProt ID
Q15910
Gene (Ensembl)
ENSG00000106462
Entrez Gene ID
Entrez gene: 2146
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-EZH2 (AMAb91749)
Anti-EZH2 (AMAb91752)

Questions?

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