Enhanced Validation

Monoclonal Anti-EMD Antibody

emerin
References (1)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human EMD
Alternative Gene Names
LEMD5, STA
Open Datasheet
Price
$505.00
Product Number
AMAb90562
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[5 pictures]
Immunohistochemical staining of human skin shows strong nuclear membrane positivity in the epidermis cells.
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[2 pictures]
Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
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[5 pictures]
Immunofluorescence staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
emerin
Target Gene
EMD
Belongs to Antibody Panel
Organelle Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000037241 (73%)

Mouse ENSMUSG00000001964 (71%)

Clonality
Monoclonal
Clone ID
CL0203
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:5000 - 1:10000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90562 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90562 on the Human Protein Atlas
Protein Name
emerin
Gene Name
EMD
Alternative Gene Names
LEMD5, STA
UniProt ID
P50402
Gene (Ensembl)
ENSG00000102119
Entrez Gene ID
Entrez gene: 2010
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Dorland YL, Cornelissen AS, Kuijk C, Tol S, Hoogenboezem M, van Buul JD, Nolte MA, Voermans C, Huveneers S

Nuclear shape, protrusive behaviour and in vivo retention of human bone marrow mesenchymal stromal cells is controlled by Lamin-A/C expression

Sci Rep , 2019 Oct 7; 9:14401. Epub 2019 Oct 7

PubMed ID: 31591420 DOI: 10.1038/s41598-019-50955-x

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-EMD (AMAb90560)
Anti-EMD (HPA000609)
Corresponding Antigens
PrEST Antigen EMD (APrEST74058)

Questions?

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