Enhanced Validation

Monoclonal Anti-CTCF Antibody

CCCTC-binding factor (zinc finger protein)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human CTCF
Price
$505.00
Product Number
AMAb90663
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
Target Protein
CCCTC-binding factor (zinc finger protein)
Target Gene
CTCF
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000017674 (100%)

Mouse ENSMUSG00000005698 (99%)

Clonality
Monoclonal
Clone ID
CL0304
Isotype
IgG2a
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Protein Name
CCCTC-binding factor (zinc finger protein)
Gene Name
CTCF
UniProt ID
Gene (Ensembl)
Entrez Gene ID
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Andersson S, Konrad A, Ashok N, Pontén F, Hober S, Asplund A

Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection

J Histochem Cytochem , 2013 Nov; 61(11):773-784

PubMed ID: 23920108 DOI: 10.1369/0022155413502360

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Questions?

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