Enhanced Validation

Monoclonal Anti-CTCF Antibody

CCCTC-binding factor (zinc finger protein)
References (1)
Recommended Applications
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human CTCF
Open Datasheet
Price
$505.00
Product Number
AMAb90663
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemical staining of human endometrium shows moderate to strong nuclear positivity in glandular and stromal cells.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
CCCTC-binding factor (zinc finger protein)
Target Gene
CTCF
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000017674 (100%)

Mouse ENSMUSG00000005698 (99%)

Clonality
Monoclonal
Clone ID
CL0304
Isotype
IgG2a
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:1000 - 1:2500

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)
All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90663 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb90663 on the Human Protein Atlas
Protein Name
CCCTC-binding factor (zinc finger protein)
Gene Name
CTCF
UniProt ID
P49711
Gene (Ensembl)
ENSG00000102974
Entrez Gene ID
Entrez gene: 10664
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Andersson S, Konrad A, Ashok N, Pontén F, Hober S, Asplund A

Antibodies Biotinylated Using a Synthetic Z-domain from Protein A Provide Stringent In Situ Protein Detection

J Histochem Cytochem , 2013 Nov; 61(11):773-784

PubMed ID: 23920108 DOI: 10.1369/0022155413502360

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-CTCF (AMAb90664)
Anti-CTCF (AMAb90666)
Anti-CTCF (HPA004122)
Corresponding Antigens
PrEST Antigen CTCF (APrEST79786)

Questions?

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