Enhanced Validation

Monoclonal Anti-CRYAB Antibody

Crystallin alpha b
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human CRYAB
Alternative Gene Names
CRYA2, HSPB5
Open Datasheet
Price
$505.00
Product Number
AMAb91661
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAb91661 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.
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[1 pictures]
Western blot analysis in human cell line U-2 OS.
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[1 pictures]
Immunofluorescence staining of U-138 MG cells using the Anti-CRYAB monoclonal antibody, showing specific staining in nuclear speckles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
Crystallin alpha b
Target Gene
CRYAB
Verified Species Reactivity
Human, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000032060 (100%)

Rat ENSRNOG00000010524 (95%)

Clonality
Monoclonal
Clone ID
CL10758
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:20000 - 1:50000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91661 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91661 on the Human Protein Atlas
Protein Name
Crystallin alpha b
Gene Name
CRYAB
Alternative Gene Names
CRYA2, HSPB5
UniProt ID
P02511
Gene (Ensembl)
ENSG00000109846
Entrez Gene ID
Entrez gene: 1410
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Did we miss your publication?

Have you published using AMAb91661? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-CRYAB (AMAb91662)
Anti-CRYAB (HPA057100)

Questions?

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