Enhanced Validation

Monoclonal Anti-CNP Antibody

2',3'-cyclic nucleotide 3' phosphodiesterase
References (10)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human CNP
Open Datasheet
Price
$505.00
Product Number
AMAb91072
Unit Size
Concentration
Lot dependent
Availability
5 in Stock
Ships
Ready to Ship
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[10 pictures]
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using AMAb91072 antibody. Corresponding CNP RNA-seq data are presented for the same tissues.
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[2 pictures]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
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[1 pictures]
Immunofluorescence staining of RH-30 cells using the Anti-CNP monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
2',3'-cyclic nucleotide 3' phosphodiesterase
Target Gene
CNP
Belongs to Antibody Panel
Neuroscience Markers
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Rat ENSRNOG00000017496 (77%)

Mouse ENSMUSG00000006782 (76%)

Clonality
Monoclonal
Clone ID
CL2887
Isotype
IgG2b
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:10000 - 1:20000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91072 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91072 on the Human Protein Atlas
Protein Name
2',3'-cyclic nucleotide 3' phosphodiesterase
Gene Name
CNP
UniProt ID
P09543
Gene (Ensembl)
ENSG00000173786
Entrez Gene ID
Entrez gene: 1267
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Kurki SN, Uvarov P, Pospelov AS, Trontti K, Hübner AK, Srinivasan R, Watanabe M, Hovatta I, Hübner CA, Kaila K, Virtanen MA

Expression patterns of NKCC1 in neurons and non-neuronal cells during cortico-hippocampal development

Cereb Cortex , 2022 Dec 27; 33(10):5906-5923. Epub 2022 Dec 27

PubMed ID: 36573432 DOI: 10.1093/cercor/bhac470

McNamara NB, Munro DA, Bestard-Cuche N, Uyeda A, Bogie JF, Hoffmann A, Holloway RK, Molina-Gonzalez I, Askew KE, Mitchell S, Mungall W, Dodds M, Dittmayer C, Moss J, Rose J, Szymkowiak S, Amann L, McColl BW, Prinz M, Spires-Jones TL, Stenzel W, Horsburgh K, Hendriks JJ, Pridans C, Muramatsu R, Williams A, Priller J, Miron VE

Microglia regulate central nervous system myelin growth and integrity

Nature , 2022 Dec 14; 613(7942):120-129. Epub 2022 Dec 14

PubMed ID: 36517604 DOI: 10.1038/s41586-022-05534-y

Sadler GL, Lewis KN, Narayana VK, De Souza DP, Mason J, McLean C, Gonsalvez DG, Turner BJ, Barton SK

Lipid Metabolism Is Dysregulated in the Motor Cortex White Matter in Amyotrophic Lateral Sclerosis

Metabolites , 2022 Jun 17; 12(6):554. Epub 2022 Jun 17

PubMed ID: 35736487 DOI: 10.3390/metabo12060554

Neely SA, Williamson JM, Klingseisen A, Zoupi L, Early JJ, Williams A, Lyons DA

New oligodendrocytes exhibit more abundant and accurate myelin regeneration than those that survive demyelination

Nat Neurosci , 2022 Feb 14; 25(4):415-420. Epub 2022 Feb 14

PubMed ID: 35165460 DOI: 10.1038/s41593-021-01009-x

Barton SK, Magnani D, James OG, Livesey MR, Selvaraj BT, James OT, Perkins EM, Gregory JM, Cleary E, Ausems CR, Carter RN, Vasistha NA, Zhao C, Burr K, Story D, Cardinali A, Morton NM, Hardingham GE, Wyllie DJ, Chandran S

Transactive response DNA-binding protein-43 proteinopathy in oligodendrocytes revealed using an induced pluripotent stem cell model

Brain Commun , 2021 Oct 26; 3(4):fcab255. Epub 2021 Oct 26

PubMed ID: 35350711 DOI: 10.1093/braincomms/fcab255

Swire M, Assinck P, McNaughton PA, Lyons DA, ffrench-Constant C, Livesey MR

Oligodendrocyte HCN2 Channels Regulate Myelin Sheath Length

J Neurosci , 2021 Sep 22; 41(38):7954-7964

PubMed ID: 34341156 DOI: 10.1523/JNEUROSCI.2463-20.2021

James OG, Selvaraj BT, Magnani D, Burr K, Connick P, Barton SK, Vasistha NA, Hampton DW, Story D, Smigiel R, Ploski R, Brophy PJ, ffrench-Constant C, Lyons DA, Chandran S

iPSC-derived myelinoids to study myelin biology of humans

Dev Cell , 2021 May 3; 56(9):1346-1358.e6

PubMed ID: 33945785 DOI: 10.1016/j.devcel.2021.04.006

Swire M, Ffrench-Constant C

Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter

Bio Protoc , 2020 Oct 20; 10(20):e3792. Epub 2020 Oct 20

PubMed ID: 33659446 DOI: 10.21769/BioProtoc.3792

Swire M, Kotelevtsev Y, Webb DJ, Lyons DA, ffrench-Constant C

Endothelin signalling mediates experience-dependent myelination in the CNS

eLife , 2019 Oct 28; 8:e49493. Epub 2019 Oct 28

PubMed ID: 31657718 DOI: 10.7554/eLife.49493

Milbreta U, Lin J, Pinese C, Ong W, Chin JS, Shirahama H, Mi R, Williams A, Bechler ME, Wang J, ffrench-Constant C, Hoke A, Chew SY

Scaffold-Mediated Sustained, Non-viral Delivery of miR-219/miR-338 Promotes CNS Remyelination

Mol Ther , 2018 Dec 1; 27(2):411-423. Epub 2018 Dec 1

PubMed ID: 30611662 DOI: 10.1016/j.ymthe.2018.11.016

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