Enhanced Validation

Monoclonal Anti-CHAT Antibody

choline O-acetyltransferase
References (2)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human CHAT
Open Datasheet
Price
$505.00
Product Number
AMAb91130
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[8 pictures]
Immunohistochemistry analysis in human placenta and liver tissues using AMAb91130 antibody. Corresponding CHAT RNA-seq data are presented for the same tissues.
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[1 pictures]
Immunofluorescence staining of SK-MEL-30 cells using the Anti-CHAT monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
choline O-acetyltransferase
Target Gene
CHAT
Belongs to Antibody Panel
Neuroscience Markers
Verified Species Reactivity
Human, Mouse, Rat
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000021919 (96%)

Rat ENSRNOG00000025012 (96%)

Clonality
Monoclonal
Clone ID
CL3173
Isotype
IgG2b
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91130 on the Human Protein Atlas
Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91130 on the Human Protein Atlas
Protein Name
choline O-acetyltransferase
Gene Name
CHAT
UniProt ID
P28329
Gene (Ensembl)
ENSG00000070748
Entrez Gene ID
Entrez gene: 1103
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Provenzano F, Nyberg S, Giunti D, Torazza C, Parodi B, Bonifacino T, Usai C, Kerlero de Rosbo N, Milanese M, Uccelli A, Shaw PJ, Ferraiuolo L, Bonanno G

Micro-RNAs Shuttled by Extracellular Vesicles Secreted from Mesenchymal Stem Cells Dampen Astrocyte Pathological Activation and Support Neuroprotection in In-Vitro Models of ALS

Cells , 2022 Dec 4; 11(23):3923. Epub 2022 Dec 4

PubMed ID: 36497181 DOI: 10.3390/cells11233923

Leyrer-Jackson JM, Holter M, Overby PF, Newbern JM, Scofield MD, Olive MF, Gipson CD

Accumbens Cholinergic Interneurons Mediate Cue-Induced Nicotine Seeking and Associated Glutamatergic Plasticity

eNeuro , 2021 Feb 2; 8(1):ENEURO.0276-20.2020. Epub 2021 Feb 2

PubMed ID: 33239269 DOI: 10.1523/ENEURO.0276-20.2020

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-CHAT (AMAb91129)
Anti-CHAT (HPA048547)
Corresponding Antigens
PrEST Antigen CHAT (APrEST86792)

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