Enhanced Validation

Monoclonal Anti-CDH2 Antibody

cadherin 2, type 1, N-cadherin (neuronal)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Genetic validation in WB by siRNA knockdown.
Product Description
Monoclonal Antibody against Human CDH2
Alternative Gene Names
CD325, CDHN, NCAD
Open Datasheet
Price
$505.00
Product Number
AMAb91220
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[6 pictures]
Immunohistochemistry analysis in human heart muscle and skin tissues using AMAb91220 antibody. Corresponding CDH2 RNA-seq data are presented for the same tissues.
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[2 pictures]
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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[1 pictures]
Immunofluorescence staining of U-251 cells using the Anti-CDH2 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
cadherin 2, type 1, N-cadherin (neuronal)
Target Gene
CDH2
Belongs to Antibody Panel
Epithelial to Mesenchymal (EMT) Markers
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000024304 (99%)

Rat ENSRNOG00000015602 (99%)

Clonality
Monoclonal
Clone ID
CL3716
Isotype
IgG1
Host
Mouse
Buffer
The antibodies are delivered in 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
The antibody solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:500 - 1:1000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Immunofluorescence in Cell Lines (ICC-IF)

Recommended conditions:

Fixation/Permeabilization: PFA/Triton X-100

Working concentration: 2-10 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91220 on the Human Protein Atlas
Western Blot (WB)

Genetic validation in WB by siRNA knockdown.

Immunofluorescence in Cell Lines (ICC-IF)

All ICC-IF characterization data for AMAb91220 on the Human Protein Atlas
Protein Name
cadherin 2, type 1, N-cadherin (neuronal)
Gene Name
CDH2
Alternative Gene Names
CD325, CDHN, NCAD
UniProt ID
P19022
Gene (Ensembl)
ENSG00000170558
Entrez Gene ID
Entrez gene: 1000
Shipping
Normally shipped at ambient temperature
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.

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Have you published using AMAb91220? Please let us know and we will be happy to include your reference on this page.

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-CDH2 (HPA058574)
Corresponding Antigens
PrEST Antigen CDH2 (APrEST87840)

Questions?

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