Enhanced Validation

Monoclonal Anti-CD40 Antibody

CD40 molecule, TNF receptor superfamily member 5
References (1)
Recommended Applications
Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.
Product Description
Monoclonal Antibody against Human CD40
Alternative Gene Names
Bp50, p50, TNFRSF5
Open Datasheet
Price
$505.00
Product Number
AMAb90905
Unit Size
Concentration
Lot dependent
Availability
>10 in Stock
Ships
Ready to Ship
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[4 pictures]
Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using AMAb90905 antibody. Corresponding CD40 RNA-seq data are presented for the same tissues.
Show all images
[1 pictures]
Lane 1: Marker [kDa]
Lane 2: Human spleen

Human Protein Atlas

View this protein on the Human Protein Atlas
Target Protein
CD40 molecule, TNF receptor superfamily member 5
Target Gene
CD40
Verified Species Reactivity
Human
Interspecies Information

Highest antigen sequence identity to the following orthologs:

Mouse ENSMUSG00000017652 (58%)

Rat ENSRNOG00000018488 (54%)

Clonality
Monoclonal
Clone ID
CL1673
Isotype
IgG1
Host
Mouse
Buffer
40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. Material Safety Data Sheet
Purification Method
Protein A purified
Notes
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.
Unit Size
100 µl
Current Lot
Produced on demand. Contact support@atlasantibodies.com for more information.
Concentration
Product Data Sheet
Download data sheet
Immunohistochemistry (IHC)

Recommended conditions:

Dilution: 1:2500 - 1:5000

Retrieval method: HIER pH6

Western Blot (WB)

Recommended conditions:

Working concentration: 1 µg/ml

Protocols
View all protocols
Immunohistochemistry (IHC)

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

All IHC characterization data in 44 normal tissues and 20 cancers for AMAb90905 on the Human Protein Atlas
Western Blot (WB)

Validated in Western blot using relevant lysates (see Western blot application images above)

Protein Name
CD40 molecule, TNF receptor superfamily member 5
Gene Name
CD40
Alternative Gene Names
Bp50, p50, TNFRSF5
UniProt ID
P25942
Gene (Ensembl)
ENSG00000101017
Entrez Gene ID
Entrez gene: 958
Shipping
Normally shipped at ambient temperature.
Storage
Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Sadeghlar F, Vogt A, Mohr RU, Mahn R, van Beekum K, Kornek M, Weismüller TJ, Branchi V, Matthaei H, Toma M, Schmidt-Wolf IG, Kalff JC, Strassburg CP, González-Carmona MA

Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells

Cancer Immunol Immunother , 2020 Nov 12; 70(5):1451-1464. Epub 2020 Nov 12

PubMed ID: 33180184 DOI: 10.1007/s00262-020-02746-x

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Join the Explorer Program

Are you using our products in an application or species we have not yet tested? Why not participate in the Explorer Program, and we will show your contribution here. If you would like to share your results with us, the Explorer Program offers a 25µl vial free of charge with your next order.

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Alternative Antibodies
Anti-CD40 (HPA031567)
Anti-CD40 (HPA031568)
Corresponding Antigens
PrEST Antigen CD40 (APrEST78053)

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